If you have this module enabled in your hardware licence, some extra commands and features for analysis of multiple binary layers over Z become available in the Binary menu.

See also: 3D Measurement.

This module is a combination of Advanced 2D Tracking and 3D Measurement.

See Tracking 3D.

This module enables users to combine all 6 dimensions (X, Y, Z, time, wavelength, large image) within a single ND acquisition experiment.

This module extends the basic OBJ TRACKING module by the ability to track binary objects and by other features.

See Tracking.

This module enables the user to perform bio-analytical tasks. The Bio Analysis module measures and analyses time-lapse and multipoint ND images (Z-stack data sets are not supported).

Several analysis modules are available - see Bio Analysis Modules.

With this module, you can capture/create FRET images, measure Calcium ion concentration or make automatic FRET calibrations.

See FRET.

(requires: JOBS Editor) This module enables the user to perform a cell-stretching experiment using the JOBS Editor module and a specialized piezo device (Physic Instrumente E 545).

The EDF module allows you to combine an existing Z-stack of images into one focused image by picking the focused regions from each frame and the pieces together.

Please see Extended Depth of Focus.

This module enables the user to define his own image analysis procedure in a simple-to-use dialog window (Image > General Analysis and Image > General Analysis RGB).

This module enables the modular tool for building image analysis recipes.

See General Analysis 3 .

This module brings the functionality of capturing and creating HDR images within NIS-Elements.

See HDR.

It is a simple-to-use acquisition and high content analysis module. Using a pre-defined template, you can acquire image data-sets, perform high content analysis and browse the resulting data.

See HCA.

This module provides an easy graphical user interface for setting and running advanced real-time (triggered) acquisition experiments.

See Illumination Sequence

JOBS is an environment aiming at complex acquisition and data analysis with easy to use and comprehensive user interface. JOBS is a “visual programming” tool enabling non-programmers to design their own automated acquisition / analysis experiments. This module allows user to create, edit and run JOBs.

See JOBS and HCA.

With this module, you can easily share the JOBS database. Typically, it is used on multi-workstation systems where one PC is used for data acquisition and another for performing analyses.

See JOBS and HCA.

This module enables user to browse the data acquired via JOBs.

See Running a Job, Viewing Results and Graphs.

Support for N-STORM acquisition and molecule analysis.

See N-STORM, View > Analysis Controls > Molecule Analysis .

An artificial intelligence (AI) module that utilizes deep learning and neural networks to automatically perform image processing tasks. The module comes with some pre-trained networks but also provides tools for training of your own neural networks.

See NIS.ai.

Motion characteristics of objects such as living cells can be measured automatically or manually using the Tracking module. Tracking is based on ROIs.

See Tracking.

Provides the ability to classify binary objects.

See Object Classifier.

Enables the Analysis > Out Proc task in Jobs.

This module is used to perform fast hardware-driven (triggered) acquisition experiments.

See Triggered Acquisition.

Single Particle Tracking (SPT) is used to track single molecules and for analysing parameters of these tracks.

Please see: Single Particle Tracking.

An artificial intelligence-based module for simple management of advanced experiments performed with the Nikon Ji microscope.

Please see: Smart Experiment.

Wound Healing analysis support.

Image deconvolution module suitable for standard 2D images. Apart from the 2D deconvolution, it provides a Fast Deconvolution and Live De-Blur (applicable on live image).

Please see: Deconvolution

Image deconvolution module suitable for image sequences containing a Z dimension.

Please see: Deconvolution

(requires: Local Option)

This module is for automated and semi-automated measurement of air void characteristics of hardened concrete.

This industrial module is aimed at analysis of filters according to the ISO 16232 standard.

See Filter Particle Analysis.

This module adds the Applications > Layer Thickness Measurement > Measure Layers command to the main menu. The module can perform different types of layer thickness measurement.

Please see Layer Thickness Measurement.

After the module installation, the Applications > Metallography > Cast Iron command appears in the main menu. Run this command to display the cast iron measurement layout.

Please see Metalo - Cast Iron Analysis.

Adds the Applications > Metallography > Grain Size command to the main menu. The command displays a layout dedicated to grain size measurement.

Please see Metalo - Grain Size Analysis.

With this module, you can:

Support for Nikon Confocal Microscope Ax.

(requires: Local Option) Support for an additional camera, it displays its live image and FFT image in a separate window.

(requires: Local Option) DMD support. E.g.: Nikon Ti-Lapp, Andor Mosaic 3, Mightex Polychrome 400.

Support for dual camera drivers (two mono cameras behaving as a single dual-channel camera). Specific camera models are supported.

(requires: Local Option) FLIM support

(requires: Local Option) Thirdparty monochromator support. E.g.: Till Photonics Polychrome V.

Nikon N-SIM support.

Offline Nikon N-SIM support.

(requires: Local Option) Thorlabs optical tweezers support.

(requires: Local Option) A plugin used for acquiring two image sources - confocal A1 and Imaging Source - to one image.

(requires: Local Option) Andor Revolution DSD confocal support.

(requires: Local Option) This module enables NIS-Elements connect and control a slide loader.

(requires: Local Option) Swept field confocal support.

Third party filter-wheels support.

Yokogawa Spinning Disc (CSU-X1) support.

Yokogawa Spinning Disc (CSU-W1) support.

Dual View and Quad View support.

See also Using a “Multiview” Device.

This module enables the user to connect and control motorized XY stages. All non-Nikon stages require this module.

This module enables the user to connect and control gas and temperature-controlling chambers.

(requires: Local Option) Support of structured-light-based devices (OptiGrid, ViCo).

This module enables NIS-Elements to send and receive TTL and analog signals via a DAQ board.

See also Triggered Acquisition.

(requires: Local Option) Support for heating stages.

(requires: Local Option) Update from Dual Camera Support. This module supports up to four mono cameras behaving as a single multi-channel camera.

This module enables NIS-Elements connect and control wavelength switchers.

See also Illumination Devices.

This module enables NIS-Elements connect and control a well plate loader.

Third party illumination device support.

(requires: Local Option) Third party galvo devices support (E.g.: Bruker miniscanner).

This module enables connect and control motorized Z drives.

If the database module was installed, the system can create and control image databases. A database can help you to efficiently organize your image archives and manage additional information related to the images.

Extra functions such as Automatic Deconvolution are added with this module. See NIS-Elements ER Package.

When Local Option is installed (see Step 2), NIS-Elements will provide some advanced features which did not pass the quality assurance procedure yet. We recommend to wait until they are released officially.

NIS-Elements supports export of results straight to an MS Excel sheet (see Exporting Results). MS Excel is not part of the NIS-Elements and must be purchased and installed separately.