(requires: 3D Tracking)
Tracking 3D objects is possible only on images having both T and Z dimensions. 3D objects which will be tracked have to be defined first. This can be done in three ways:
Threshold the image using Binary > Define Threshold
and then connect 3D objects using Binary > Connect 3D Objects in Binaries or run the View > Analysis Controls > Binary Layers 
tab, select your binary layer and click the 
button. For each T frame, objects touching at least with their corners will be connected together.Use Binary > 3D Spot Detection > Bright Spots or Binary > 3D Spot Detection > Dark Spots
See: Spot Detection for more details.
Once your 3D objects are defined using one of the methods listed above, run View > Analysis Controls > Tracking 
, display the Tracking Options dialog window by clicking the 
Tracking Options... button, define the tracking parameters (see: Tracking Options) and click . 3D objects are then connected through the whole timelapse and their tracks are visualized.
Figure 1602. Tracks of two 3D cells displayed in Volume View
Measured features are visualized in the Graph tab and all numeral results including 3D measurement features are listed in the Data table (see: Graph, Data and Tracks tabs).