Output of the STORM method results in a list of molecules (seeds) which can be imported (from a *.roi file) and displayed in NIS-Elements. The *.roi file contains 6 tab-separated columns for each molecule (row) in the following order: Number, X [nm], Y [nm], Z [nm], Frame number, Intensity.
| Roi file example | |||||
|---|---|---|---|---|---|
| 1 | 1403.3 | 24607 | -82.873 | 9431 | 97407 |
| 2 | 1661.7 | 22094 | -264.32 | 9485 | 91303 |
| 3 | 2519.2 | 21886 | -193.16 | 9481 | 90851 |
| 4 | 2929.6 | 23406 | -81.985 | 9467 | 90449 |
| 5 | 1537 | 25617 | -427.35 | 9426 | 90449 |