Real Time Tracking Using XY Stage

(requires: Advanced 2D Tracking)

A motorized XY stage can be used to keep the current scene - such as a living specimen - in view. This is the recommended procedure:

  1. Run live camera image and get the object of interest to the view.

  2. Click the placed on the image tool bar and specify settings for the tracking algorithm:

    Track Object Given by Rectangle

    Lets the user to draw a rectangle around an object of interest. This part of the live image will be used to track the changes.

    Keep Current Pattern in Center

    Selects a small portion of the scene in the middle.

    Create ND Timelapse

    If selected, a timelapse image sequence is created as a background process. The sequence is opened once the tracking function ends.

    Channel For Tracking

    Select the image channel most suitable for the selected tracking method (e.g. a channel having the best contrast).

    Tracking Method
    Correlation

    Searches for similarities/changes in the image data. It is suitable for any scene.

    Threshold

    Searches movement by analysing auto-thresholded binary objects. It is suitable for scenes with enough contrast.

    Spot Detection

    Searches movement by analysing auto-spot-detected binary objects. It is suitable for scenes containing circular objects.

  3. Confirm the settings by the OK button and run the turn the function by the Track XY button in the tool bar.

    Note

    If the user-defined rectangle shall be defined, the icon changes to indicate this . If the tracking algorithm fails in this mode, press Esc to exit.

  4. The function is aborted if either the user stops the tracking by the toolbar button or the scene changes rapidly so that the tracking algorithm fails.