Tick Advanced Settings.

The Percentile Threshold is the rescaled brightest pixel value that can be considered as a detection. Set to a value around 10-20%.

The Motion Blur size gives the maximum and minimum size for a detection to be accepted. A low percentile threshold shall be compensated by a high motion blur size. Set to a value between 0.1 and 2 µm².

Figure 1457. 

SNR ratio is the overall signal to noise ratio estimated of the particle. This is defined by the formula:

Figure 1458. 

The sum is computed over the pixels of the detection. The noise and variance are respectively the time median and variance of the one dimensional time series pixel without detections. SNR ratio shall be higher than 0.1.

The tracking result preview shows the density of detections in the first 500, middle 500, last 500 frames of the movie. The density shall not vary significantly unless the final statistics will be altered. The problem of error and miss-assignment.

Figure 1459.