Confocal Microscope AX | NIS-Elements

Confocal Microscope AX

Introduction

AX Hardware Configurations
Startup

Experiment Settings

Window Overview
About Experiment Presets
Duplicating an Experiment Preset
Editing a Preset
Selecting Your Favorite Fluorescent Dyes
Experiment Setup Edit Mode - Reference
Experiment panel

Multichannel and Spectral Experiments

Creating a Multichannel Experiment Preset
Editing a Multichannel Preset in Expert Mode
Creating a Spectral Experiment Preset

Multiphoton Experiments

Creating a Multiphoton Experiment Preset - DUX Spectral Detection
Creating a Multiphoton Experiment Preset - NDD Detection
Creating a Multiphoton Experiment Preset - NSPARC Detection
Changing of Filters in the NDD detector

Automatic Live Functions
Scanning Settings
Channel Settings
Pinhole Size
IR Laser Control
NIR Laser Control
NSPARC Control

AX Scan Area

Window Overview
Specifying a Custom Rectangular Area
Specifying a ROI Scan Area
Specifying a Polyline (1DT) Scan Area
Applying Shading Correction

Device Setup

Acquisition
General
Alignment
Sampling
Pinhole
Piezo
AutoSignal.ai
AutoFocus
AX-MP
Defaults
Defaults-MP
IO
NSPARC
System Info

AX + Ti2-E Automatic Correction Collar

This manual describes how to control the Nikon Confocal Microscope AX via the NIS-Elements imaging software.