LUTs is a useful tool for image color and brightness modifications. You can use LUTs to enhance images for observation purposes so the color modifications will be non-destructive to the image data. LUTs settings are saved along with the image file. If required, the LUTs settings can be applied to the image data by pressing the button. Use the
Show LUTs window button in the image toolbar to display the LUTs window.
The following actions are available within the LUTs window:
Adjusting the Gamma parameter. The gamma curve is drawn in gray over each graph. You can drag the middle point and move it up or down, or enter an exact value to the G: field at the top of the graph area.
Adjusting the input intensity range. The input intensity range can be restricted by moving the black and white triangular sliders to the center. All pixels with values outside of the modified input range will be set to maximum/minimum values and the remaining pixels will be approximated in order to fill the output (full) range.
Note
This is the way how images can be equalized. For example, if the image is very dark and most of the histogram is located to the left while the right part of it is flat, you may move the white slider(s) to the position where the histogram line starts to rise. This will incredibly brighten the image and reveal details which were previously hidden in a dark.
You can adjust the histograms height non-proportionally by moving the slider on the left side of the window up and down.
Tips
Right-click the graph area and (de)select Draw trend style. When ON, the LUTs curves will be smoothed to display the data trends rather then represent the actual image data values.
The position of the black, white and Gamma sliders can be reset by a double click.
Different controls will be available when applying LUTs to monochromatic, RGB, multi-channel or spectral image. When LUTs are active, the LUTs button in the top-left corner of the image window is highlighted red.
There are 3 separate windows for each of the RGB channels. All channels are controlled at the same time by default. To control each channel separately, press and hold the Shift key while you drag the sliders or adjust Gamma.
LUTs Tools
Brightfield Opacity - You can change opacity of a Brightfield channel. Define the opacity in the Brightfield Opacity dialog window that appears after you run this command.
Use Black Level - Check this item to ensure that the black slider will be affected by the auto scale functions.
Settings - opens the Auto Scale Settings window. See Auto Scale Settings.

This button applies LUTs to the current image.

Press this button to run the auto scale procedure permanently (on the live image). When you turn this button OFF, the settings remain as if the Auto Scale button was pressed only once.

This button adjusts the white slider position of all channels automatically with the purpose to enhance the image reasonably. If you have selected the Use Black Level option from the Settings pull-down menu, the black slider will be affected too.

Discard all LUTs settings and turn LUTs OFF by pressing this button.
Display the pull down menu and select one of the following commands:
Check this option to switch the Auto White Balance functionality permanently (on the live image).
Press this button to perform the Auto White Balance operation once.
This button Opens the AWB Color window and enables you to select a color shade which the system shall eliminate (make white). See AWB.

If Keep Auto White Balance or Auto White Balance function is applied, this button turns it off.

Switch this button ON and the system highlights all pixels with values reaching maximal values. Select colors to highlight the pixels with in the pull-down menu. See also Channel Coloring.


These buttons arrange histograms of single channels next to each other horizontally or vertically.

This button arranges histograms of single channels overlapped. Use sliders at the bottom of the graph window to amplify or reduce the green/red/blue component display in the view.

This pull-down menu enables user to handle LUTs settings in various ways. They can be saved to a *.lut file and loaded later. Or, LUTs settings saved along with an image can be loaded directly from this image by the Reuse LUTs from File command. Or you can copy/paste the settings within the documents opened in NIS-Elements.
Press this button to apply the LUTs settings to the image data - the original image will be overwritten. Until you press this button, no changes are made to the image data.

Zooms the histogram to fit the preview window.

Zooms the histogram so that the “high” and “low” limits are distinguishable. For example, if a small intensity range is defined on a 16bit image the low and high lines are displayed as one-pixel line. Pressing this button will stretch the histogram in order to display the lines separately.
Press the arrow next to the Reset LUTs button and a pull-down menu appears. Invoke the Settings command.
The Low and High fields determine how many of all pixels of the picture are left outside the sliders when Auto Scale is applied (0-10%).
White color in some images may suffer from having a color tint. The AWB (Auto White Balance) mode adjusts the image to get pure white instead of this tint. Similarly to LUTs auto scale, the AWB function can be used once, or permanently on the live image. If you know the tint your “white” color has, you can select this color by the color picker that appears after pressing the ... button:
Choose the color by mouse. You can also enter the RGB values to the fields below. A preview of the chosen color appears in the rightmost rectangle.
All features mentioned above are also valid for monochromatic images, except that the AWB function is not available. There are some other features added when in “mono” mode.
Mapping monochromatic images to pseudo-color gradients is often used to enhance details in the image which would otherwise not be obvious. This button indicates the currently selected gradient. When pressed, a pull-down menu appears where you can select the color scheme you would like to use. Try a few of the schemes to see which one suits best.
The Manage Custom LUTs command in the pull down menu defines a custom LUTs gradient. Use the tools available in the dialog window to define your custom gradients. They then appear in the pull down menu ready to be applied. Please see View > Image > LUTs > Create Custom LUTs for details about the custom LUTs definition.
Most of features mentioned above are also valid for multi-channel images. There are some other features added when in “multi-channel” mode. Each channel is controlled separately by default. To control all channels at the same time, press and hold the Shift key while you drag the sliders or adjust Gamma.
When the image contains up to three channels, all channels can be visible at the same time and you can handle them similarly to the RGB mode. When there are more than three channels, only one channel is displayed at a time. A pull-down menu in the toolbar enables you to select the channel to be displayed.
Next to the channel selection pull-down menu, there is this Auto Scale button. By pressing it, the system will automatically adjust only the current channel settings.
When observing a spectral image, the graph inside the LUTs window does not show the image histogram(s), but reveals another information about the image. Intensity values are on the Y axis and spectra is on the X. There are two curves in the graph. The top (white) curve displays the maximal intensity and the color curve displays the mean intensity of each channel.
Note
Spectral image consists of many channels, each representing a single wavelength.
The LUTs behavior depends on the selected displaying mode. There are four displaying modes. You can select the mode either by a pull-down menu in the LUTs toolbar, or within the image top toolbar.
True Color
Custom Color
Grouped
Gray Scale
In the bottom part of the LUTs window, Brightness and Black Level settings can be adjusted either for all channels together or for each group separately - depending on the displaying mode.
Note
The Brightness and Black Level sliders offer a range of values to be set by moving them, but any higher value can be entered in the field next of the sliders.
TD channel
A spectral image can contain additional TD (transmitted detector) channel. Its basically a standard monochromatic image. LUTs settings of this channel appear on the right edge of the LUTs window.
True Color Displaying Mode
You can switch to this displaying mode by clicking the button located in the image toolbar.
Custom Color Displaying Mode
You can switch to this displaying mode by clicking the button located in the image toolbar. You can assign custom colors to sections of the spectrum. Sections (colors) can be added/removed by clicking the + and - buttons placed below the spectral graph. To change the section color, click the button located in the color stripe. A window appears where you can select the color from a palette or define it by Hue or Wavelength settings.
Grouped Displaying Mode
You can switch to this displaying mode by clicking the button located in the image toolbar. This view enables you to reduce the number of channels multiple-times. You can adjust the number of groups by the + and - buttons and also change each channel width by moving the white horizontal dividers. Brightness and Black Level for each group can be adjusted.
Gray Scale Displaying Mode
You can switch to this displaying mode by clicking the button located in the image toolbar. The image is displayed in gray scale, but various color gradients can be applied to it.