The defined directory is always used when using the File > Open or File > Save As command.

If checked, only one document at a time can be opened.

Limit the number of images, which can be opened at one time. The maximum is 128 opened images.

If checked, every acquisition creates a new image.

When a new image is opened, this option turns the look up tables automatically on, and copies the settings from the current or the last opened image. Some image formats (JP2, ND2) can contain the LUTs settings. If such image is opened, the saved LUTs are loaded instead of the most recently used.

This option keeps the AutoLUTs feature of the File > Open window always ON, and it applies AutoLUTs to the image after opening. This option is ignored if LUTs settings are saved in the image, or if the Use last LUTs on image open option is applied.

Displays the Mapping window every time an image is inserted to a database table. It enables you to check/modify mapping of the image info values to the database table fields.

If Single TIFFs option is selected as the output format in the ND Acquisition window, here you can specify how the single frames of the ND experiment are saved into a TIFF file. Choose one image format (Mono image for each channel, Color image for each channel or Channels merged into one color image) and set the order of the dimensions in the Index order section. Images can be optionally converted to 16-bit (Save 10, 12, 14-bit images as 16-bit) and multichannel images to an RGB image (Convert multichannel images to RGB).

Displays each newly generated ND view in a separate window.

Authentication verifies each image frame and detects changes in image data made after acquisition. Modifications are indicated in the Image Properties dialog. See File > Image Properties.

Displays the File > Image Properties window every time an image is saved via the Save As command.

When you select to open an image that is a part of image sequence such as 001.JP2, 002.JP2..., it is recognized automatically and you are offered to convert it to an ND2 file.

Check this item to display contours of binary layers.

Select this option to ensure that the first frame of a time sequence will always start at 0.0s.

Select what to do with the saturation indicator settings () after NIS-Elements is restarted. Turn it ON, OFF, or remember the last setting.

If checked, in overlay mode both binary and color images are rotated, shifted and flipped.

Make changes to the current optical configuration without warning? If selected, some setting changes are written to the optical configuration immediately and the exclamation mark would not appear by the OC name. Select the settings to be changed without warning:

Enabled if “Unselect” in the option below is chosen.

Allows NIS-Elements to automatically change the optical configuration based on the filter or nosepiece selected on the microscope.