User Manual

Table of Contents

Startup
Command Line Startup Options
Installation and Settings
Installation and Updates
The Installation DVD-ROM Content
NIS-Elements Installation Steps
Command Line Installation Options
Additional Module/Device Installation
Sample Database Installation
Software Copy Protection
Device Updates
Fixes
Additional Modules Available in NIS-Elements BR
Acquisition and Analysis Modules
Industrial Modules
Device Modules
Other
User Rights
NIS-Elements authentication
Creating a Shared Layout
Closing Another Instance of NIS-Elements
User Rights Options
Users Tab Options
Groups Tab Options
Privileges Tab Options
Usage Statistics
NIS-Elements Preferences
Adjusting Program Preferences
General
Hardware licences
What is a hardware licence?
How do I find out the content of my USB key?
Is there an update for my software?
What is SUA?
User Interface
Introduction
Compact Layout
Compact Layout Options
LUTs
Analysis Panels
Acquisition Panel
Image Window
Advanced Layout
Main Window Components
Image Window
Arranging User Interface
Modifying Layouts
Layout Manager
Modifying Toolbars
Modifying Menus
Running a Macro Upon Layout Change
Appearance Options
Quick Context Menu
Handy Key Shortcuts, Mouse Wheel Options
Handy Keyboard Shortcuts
Mouse Wheel
Cameras & Devices
Device Manager
Key Features
Hierarchy of Configurations
Startup Hardware Configuration Selection
Device Manager Window
Adding Cameras and Devices to Hardware Configuration
Connecting a Device to NIS-Elements
Lightpath Scheme
What are Logical Devices?
Objectives
Managing Objectives
Assigning Objectives to Nosepiece Positions
Objective Calibration
Manual Calibration
Objective Calibration Using XY stage
Monitor Calibration
Adding Objectives to Objective Database
Control of Motorized Devices
Controlling Illumination Devices
Motorized Stage Initialization
Setting Software Limits to Stage Movement
Using two independent Z Drive devices
Fast Auto Focus and Continuous Scanning
Camera Settings
Optical Configurations
Introduction to Optical Configurations
Creating New Optical Configuration
Managing Optical Configurations
Operations with Optical Configurations
Using Auto Exposure while Switching between Optical Configurations
Cameras
Nikon DS-Fi3
Nikon DS-Qi2
Nikon DS-Ri2
Nikon Digital Sight 10
Nikon Digital Sight 50M
Andor Ixon Ultra 897
Andor Neo
Andor Zyla
Generic HDMI
Imaging Source USB Industrial Cameras
Photometrics Prime 95B
Hamamatsu ORCA
Using Probe for AE / AWB
Troubleshooting - Blooming, Smearing
Microscopes
Nikon Ci-E Microscope
Nikon AZ100 Microscope
Nikon Eclipse LV Series Microscope
Nikon Eclipse L200N(D)/L300N(D) Microscope
Nikon Eclipse MA200 Microscope
Nikon MM 400/800 Microscopes
Nikon Ni-E Microscope
Nikon Ni-U/Ni-L Microscope
Nikon SMZ Stereomicroscopes
Nikon Ti2-E Microscope
Nikon Ti2-A Microscope
Nikon Ti2-U Microscope
Illumination Devices
Nikon Intensilight
Nikon C-LEDFI
Nikon D-LEDI
Nikon LU4A
Nikon LUA-S
Nikon LUD-H
Nikon LU-N3/4/4S
Nikon LU-NV
Nikon LUSU
Agilent MLC400
AOTF via NIDAQ - Illumination Device
CoolLED PrecisExcite
EXFO X-Cite Exacte
Sutter Lambda DG-4 / DG-5
Sutter Lambda XL
Lumen Dynamics X-Cite 120
Lumen Dynamics X-Cite XLED1
Lumencor LIDA
Lumencor SOLA
Lumencor SPECTRA / AURA
Photonic F3000
Schott KL 2500 LED
TILL Photonics Polychrome V
89 North Heliophor
Other Devices
Andor Mosaic 3
CrEST X-Light
Incubator
Nikon Ti-LAPP system
NII Correction Collar
Legacy Cameras and Devices
Nikon DS-U3/L3
Nikon DS-U3/L3 - Qi1
Nikon DS-U3/L3 - Ri1
Nikon DS-U3/L3 - Vi1/Fi1/Fi2
Nikon DS-U2/L2
Nikon Eclipse 90i/80i Microscope
Nikon TE2000 Microscope
Nikon Ti Microscope
Image Acquisition
Experiment Acquisition
Basic image acquisition
Lightpath tool bar
Image acquisition via optical configurations
Multi-channel image acquisition via experiment settings
Experiment pad
Adjusting and managing experiments
Sample navigation
Service settings
Camera ROI
Shading Correction
Simple Shading Correction
Shading Correction per Optical Configuration
Removing Dust from Camera Chip Using Shading Correction
Shading Correction Panel
About ND Acquisition
Introduction to ND Acquisition
Common ND Experiment Options
ND Sequence Options
Time-lapse Acquisition
Special Options
Multi-point Acquisition
Point by Point (Manual) Multi-Point
Well Plate (Rectangular) Multi-Point
Large Image (Covering) Multi-Point
Random Multi-Point
To Change a Single Z Coordinate
Special Options
Z-series Acquisition
Multi-channel Acquisition
Large-image Acquisition
Combined ND Acquisition
Save to File
Recorded Data
Speeding Up ND Acquisition
Experiment Timing
ND Experiment Progress
File Simulator Setup
Displaying Images
Open, Save, Close Images
Opening Image Files
Saving Image Files
Closing Images
Supported File Formats
Floating Point Images
Open File Formats
Image Layers
Introduction to Image Layers
Image Types
Channel Coloring
Assigning Colors to Channels
Copying Channels by Drag and Drop
Shifting Image Channels
ND Dimensions
Navigation in ND2 Files
ND Views
Synchronizer
Volume Viewer
Basic Operations
Opening large volume datasets
Time-to-Volume Projection
Volume Options (Standard renderer)
3D Settings
Large Images
Handling Extra-Large Image Files
About Multi-Resolution Images
Creating a Multi-Resolution Image
Splitting Large Images
Locating XY Positions Between Images
Methods Used for Stitching Large Images
Image Sequence Description
LUTs - Non-destructive Image Enhancement
Introduction to Look-Up Tables (LUTs)
LUTs on RGB Images
LUTs on Monochromatic Images
LUTs on Multichannel Images
Organizer
About Organizer
Image Filter
Operations with Images within Organizer
Resizing the Organizer Panes
Reporting
Image Tags
Image Analysis
Preparation
Histogram
Image processing
Image Segmentation
Thresholding
Binary Layers
Introduction to Binary Layers
Managing Binary Layers
Binary Editor
Mathematical Morphology Basics
Regions of Interest - ROIs
Introduction to ROIs
ROIs in Time - Moving ROIs
Measurement
Calibration
Introduction
Calibrating an (Uncalibrated) Image
Units
Indicative Measurement
Object Count
Thresholding the Image
Applying Restrictions
Results
Object Count Procedure Example
Measurement Options
Measurement on Graph
Pixel Classifier
Manual Measurement
Basic Workflow
Working with the Measurement and Annotation Objects
Measurement Tools
Automated Measurement
Time Measurement
Quick Guide
Time Measurement Tools
Options
Measurement Features
Exporting Results
Export Destinations
Data Export Options
Creating Reports
Report Generator
Report Objects
Report Templates
Macros
Creating Macros
Running a Macro
Macro Language Syntax
Variable types
Structures and Unions
Arrays
Local and Global Variables
Statements
Directives
Operators
Priority of Operators (!)
C-like functions
Controlling Cameras by Macro
Macro Preferences
Movies
Capturing AVI Movie
Save ND2 as AVI
Creating a Movie from Volume View
Creating a Movie from EDF Surface View
Creating a Movie Step by Step
Movie Maker Controls
Directors Mode
Special Options
Presets
About Video Compression
Troubleshooting
How to send LOG files
Application LOG files
Devices communication log files
Cameras communication log files
Nikon SDK log files
Displaying hidden files in Microsoft Windows 10
Determining COM port numbers
HP Z440/Z840 Power Management
Introduction
BIOS Settings - HP Z440
BIOS Settings - HP Z840
BIOS Settings of Other PC Models and Manufacturers
Windows Power Options
Disabling C-States via Windows Registry
Restoring C-States via Windows Registry