(requires: Stage)
There is a special option in the ND2 acquisition window, to capture a Large Image. A motorized XY stage is required to have this option enabled. When set to work, it captures several frames within a defined area and stitches them together, so you get an extra large image.
NIS-Elements offers multiple ways how to capture a large image:
The most complex large image acquisition.
Custom large image acquisition for manual area selection.
A tab inside the ND Acquisition window used when the area of interest exceeds the camera FOV.
Addons > Large Images > Scanning Wizard  |
Addons > Large Images > Scanning Wizard EDF  |
_LargeImageGrabbingFreeShape A large image created by stitching (obsolete function only executable by a macro).
_LargeImageGrabbing Large image grabbing (obsolete function only executable by a macro).
Note
When camera ROI on Live Image is on (see: Camera ROI), only its frame defining the sensor area is used during the scanning.
Figure 178.
The area can be defined in three ways:
Check the first radio button and define the number of rows and columns of the scanning grid.
Check the second radio button and define the size of the scanned area in millimeters.
Click the button and select an XML file containing a large-image pattern.
This XML can be created using the _LargeImageGrabbingFreeShape macro function.
Caution
Do not mistake this XML with similar XMLs created by other functions. For example, XML files saved via the Acquire > Capture Multipoint > Capture Automatically window will not work here.
Define the stitching method:
Enter the percentage of image overlapping which is used for image stitching.
If checked, the system automatically stitches the images together to form a large image using a precise stitching algorithm (image registration). If there are more channels available, select which one to use from the pull-down menu (Use).
If unchecked, the system will stitch the images together with the defined overlap using the recorded XY coordinates but without using the Image Registration algorithm. Stitching can also be done manually after the acquisition finishes via the File > Stitch Large Image from Files command.
The active shutter will close in times between acquisitions phases. Typically, this option is used to reduce the photo-bleaching effect on live cells.
If checked, the Perfect Focus System (PFS) is utilized. A new “PFS” column is shown in the table with a button filling in the PFS value. Context menu over the value enables the user to Offset All Points in PFS or to Reset All PFS Offsets which sets the same (current) offset for all points.
Automatic focusing can be used during the experiment. You can select the auto-focus method that best meets your needs. The Define button shows a window where you can define parameters of the selected focusing method.
Focuses using the surface defined in the Focus Surface tab of the View > Acquisition Controls > XYZ Overview 
panel.
This delay between captures can be used for sample stabilization, e.g. after XY movement or autofocus.