This example combines GA3 and Jobs to analyze each well of a wellplate, mark wells with few or none cells as empty and never visit them again.

Start by defining the general analysis. Find bright spots in the live image on a sample well where cells are present. Then drag the Measurement > Whole field > Object Count onto the found spots to count the number of cells. Save this GA3 recipe and switch to Jobs.

Figure 895. Analysis definition.

Figure 896. Detected cells in a well.

Create a new Job - define the used well plate (Well Plates > Define Plate), align it (Well Plates > Align Well Plate Holder), select the range of wells to be observed (Well Plates > Select Wells) and insert the first Well Plates > Loop over Wells. Put an System > Expression inside the loop and write down the condition assigning class “1” to each visited well.

Then add Time Series > Repeat N times to repeat the loop twice and place another Well Plates > Loop over Wells inside it. Then move to a center of a well (Well Plates > Move to Well Center), capture an image of the well with the current optical configuration (Acquisition > Capture Current OC) and execute the analysis previously set in GA3 (Analysis > General Analysis) with “ObjectCount” as an Export Parameter. Finally evaluate the condition (Conditions > Condition (If)) so that all wells with less than 10 cells are marked with a class “0” by the System > Expression.

Figure 897. JOBs definition.