Main Toolbar
Create new document - creates a new image document once the analysis run is finished.
Apply only on current frame - performs the analysis just on the current image frame.

Creates a new empty analysis.

Saves the current analysis.

Saves the current analysis into a recipe with a given name and notes.


These buttons implement a standard undo/redo functionality.

Imports an analysis from a .ga3 file.

Exports the current analysis into a .ga3 file.

Exports the current analysis into a .html file.

Brings the image being processed forward.

Opens the Crop preview document where the size and dimensions of the current image can be cropped. The user can enter numeral values of the crop or directly resize and move the red rectangle in the image and select a loop range of the document. Use the Crop dimensions slider to set the dimension range or use Ctrl + (Shift) + click to select the document frames for the preview (highlighted green) and click the secondary mouse button to confirm the selection. Once the crop is confirmed by clicking , a new cropped document is opened.

Opens the Edit Wizard dialog window used for adjusting and activating nodes being marked Show in wizard. After clicking the Edit Task Description button, the description of each node and any inserted thumbnails can be adjusted using the top toolbar tools or using the HTML View (HTML 4 markup).

Opens the help file.

Preview ROI is used for previewing purposes to speed up the analysis definition. It defines a region of interest in the Define preview ROI dialog window or directly by resizing and moving the red rectangle in the image and selecting a loop range of the document. Use the ND dimensions slider to set the dimension range or use Ctrl + (Shift) + click to select the document frames for the preview (highlighted green) and click the secondary mouse button to confirm the selection. Information about the ROI selection is shown next to the button. To reset the ROI to the full size/full frames of the original image, click on Delete preview ROI.
Switches the node list from 2D to 3D nodes.
This search box can be used to quickly find a node. Type in the name of a node, select it from the list of found results and click on it. It is then highlighted in the list below.

Shows/hides the task panel. Hiding the panel is useful for observing more complex analyses.
If turned on, a preview of the analysis is shown in the processed image. Progress bar is shown until the preview is generated.

Performs the defined analysis on the linked image.

Reveals two run options:

Any custom comment can be created using the Rich text editor and added to the graph area .

Adjusts the zoom factor to view the graph in its original size.

Zooms the graph to fit in the window.
Context menu over a node

Turns the node on/off. Disabled nodes are not used in the analysis.

Deletes the selected node.

Opens the help of the specific node.

Highlights the selected node in the node list.

Renames the selected action.
If two and more nodes are selected, they can be merged into a single function using this command. The inner nodes can be edited (Edit Function) or restored back (Decompose Function). Please see Functions, groups and sections for details.
Groups all selected nodes together. To ungroup the actions, click Decompose Group.

Marks the selected action with a symbol and puts it into the GA3 Wizard which is shown just after clicking . Once you go through all steps of the wizard and adjust parameters of all the actions, click to execute the analysis.

Duplicates the selected action.

Adds more table inputs which can be used to refine the action and filter its output. For example find Segmentation > Spot detections > Bright Spots on a channel, add a dynamic parameter “P1: Spots.Diameter” and connect it to records from a different measurement containing EqDiameter data. The Segmentation > Spot detections > Bright Spots action is refined and the outcome respects the EqDiameter information.
To add a dynamic parameter check the first check box in the Dynamic Parameters table and a new input with its name is shown above the node.
Context menu over a result


Defines whether the selected result is saved or not after the analysis is run.

Can be used to add custom description to the selected result or use the default one.
Selects all nodes in the layout.
Can be used for changing color of the selected result.
Can be used to move the selected result above or below other results so that the preview and outcome can be properly seen.
A new channel is added to the source image.

Renames the selected result.

Creates a new binary layer which can be viewed in the Binary Layers (View > Analysis Controls > Binary Layers ) panel.

Takes the binary layer of the current node and moves it into the selected layer.

Changes the order of the binary layer of the current node.
Record Options
Each record is represented by a complex table. The following options are available:

Results are shown as tabs (only one result is visible at once).

Results are shown at once splitting the Analysis Results window.

Results are splitted horizontally (e.g. table data are shown next to their graph interpretation).

Results are splitted vertically.

Summary statistics for all records in the table are shown at the bottom.

Exports the current records to MS Excel or Clipboard. Choose an option from the drop-down menu.

The binary object selected in the table is highlighted in the image after clicking this button.

Enables the user to select which columns are shown in the records table, change their name, format, and precision.

Turns on all the tracking features set in the tracking Settings... found under the drop-down arrow. These features help visualize objects tracked using Tracking nodes.
For more information about tracking capabilities, please see Tracking.