If spot detection is used for segmenting the nuclei but the Grow function is not used, the detection of the cell border may be problematic.

Figure 773. Original image.

Some inner nuclei/cell border areas may occur (red arrows).

Figure 774. Detecting errors.

Proper cell detection should start by detecting the precise border of the nuclei (e.g. by using Bright Spots (3D Segmentation > Spot detection > Bright Spots) with the Grow function checked.

Figure 775. General analysis definition.

Figure 776. Precisely detected nuclei using the spot detection with Grow.

Then another grow (Binary processing > Region growing > Grow Regions) is applied using a channel carrying the cell border information.

Figure 777. Precisely detected cells