Analysis definition with the source image can be downloaded here.
Sholl analysis is a method of quantitative analysis used in neural studies to compare and characterize the morphology of different types of neurons.
Figure 687. Original image
Figure 688. Analysis definition
Our example has five main steps as shown in the analysis definition above:
Enhancing the image using local contrast.
Detect the whole neuron by thresholding to accurately recognize the number of dendrite branches with increasing distance from the neuron body.
Creating spherical shells and their dilations.
Highlighting intersections of the dendrite branches and the drawn circles and adjusting spherical shells dilation.
Fine-tuning the final line chart to display a curve showing the circles and intersections counts.
Figure 689. Binary layer
Figure 690. Resulting line chart