GA3 Processing

GA3 Processing > General Analysis 3

To utilize more powerful analyses in the JOBS Editor module on captured images, use the advantage of the GA3 Processing > General Analysis 3 task. The presence of resizing tools and optimized preprocessing features speeds up the detection and improves the overall data analysis. GA3 can also be run on an already acquired job run.

Requires:

Note

Importing of tasks containing a GA3 recipe automatically exports the included GA3 definition into the Image > Analysis Explorer.

Main Advantages

  • GA3 can be created and edited directly from Jobs.

  • One frame with relevant dimensions can be captured to serve as a preview.

  • GA3 Processing > General Analysis 3 task can be shown in the wizard mode both before the jobrun or during runtime.

  • Wizard in Jobs shows only the GA3 wizard part of the dialog. What will be visible in each wizard page is defined by the Visible Channels/Binaries/Tables nodes in the GA3 Results tab.

  • Result binaries can be exported to generate point sets.

  • Result binaries can be exported to generate region lists.

  • Result color images or binaries can be used as a stimulation pattern.

  • Result data tables can be exported as JOB parameters.

Results 
Result tables are internally stored in a HDF5 format (accessible via free external viewers).

If the result table produced by the GA3 task can be classified as a frame table (contains all required loop index columns) or object table (contains all required loop index columns, binary object ID column and entity column), it is shown together with other jobrun features in the jobrun result window view elements (grid, wellplate view, ...).

Other tables are accessible in the Analysis Results window element invoked from the jobrun result window toolbar.

Runtime Wizard 
When the GA3 Processing > General Analysis 3 task is set to be Shown at Runtime, user can adjust or define GA3 analysis settings on captured samples during the acquisition. The Show at Runtime property can be conditional, the activation/deactivation can be controlled by an System > Expression, Conditions > Condition (If) or Conditions > Condition (If-Else) tasks.

GA3 Processing > Average

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Average.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Best Focus Plane

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Best Focus Plane.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Integrate

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Integrate.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Max IP

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Max IP.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Median

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Median.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Min IP

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Min IP.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Align

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Processing > Align.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Equalize Intensity

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Processing > Equalize Intensity.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Stitch

Processes an image sequence by the following processing node of the General Analysis 3 module: ND Processing & Conversions > Stack reduction > Stitch Multi Points.

Requires:
Capture Task

Select a Acquisition > Capture placed inside a loop.

GA3 Processing > Clear Temporary Data

This task clears temporary data shared by several instances of General Analysis 3 tasks if they contain recipes with specific GA3 nodes supporting such sharing (e.g. Tracking).

It enables the user to share such data inside an inner loop (e.g Timelapse) and clear them when outer loop (e.g. Multipoint) moves to the next position.

(requires: General Analysis 3)