A simple analysis (or a complex one) can be performed either:
Inside a job using analysis tasks. E.g. to make a decision what to do next based on the analysis results - see: Using Conditions.
On data acquired by a job (see: Analysis performed on job results).
On any opened image (see: Analysis Explorer).
There are several predefined analysis types. No matter what type you choose, the top part of the definition window is the same for all of them:

This button acquires a single image according to the current Capture definition settings. Use this button to preview results of your analysis setting. By altering your parameters (such as preprocessing, thresholding, ...) a binary image is modified within the captured image.

Opens the Wellplate Navigation window where you can overview your well plate and navigate to a desired well stage on a selected well plate.

This button saves your recipe to the recipe database. The recipes can then be managed via the HCA/JOBS > Presets > Manage Recipes command.

This button loads your recipe from the recipe database.

This button opens a dialog for importing recipe files from your hard drive.

This button opens a dialog for exporting recipe files to your hard drive.

Opens the Load Job Recipe dialog window used for loading job recipes.

Opens the HTML Help about the analysis being set.
Analysis name will denote the particular analysis in the result view. Analyses with the same name are considered to be the same and thus available for aggregation.