ROIs

ROIs >

Define ROI

This task defines ROIs on the live image using the specified optical configuration.

Draw ROIs inside the live image and stimulate them using the connected stimulation device.

Task Name

Name of the Define Stimulation ROI task.

Create New ROI Definition

Creates a new Stimulation ROI Definition.

Redefine ROI from

Redefines the existing Stimulation ROI Definition from the selected point/well loop/ROI from analysis.

Use OC

Specify the optical configuration which will be used for defining the ROIs.

Draw ROI (s)

This button runs the Live view and opens the Simple ROI editor (see: ROI > Simple ROI Editor).

Define ROI(s) in Runtime

If checked, this feature enables the user to define ROIs during runtime.

Use Last Frame from Preview

If checked, the last frame from the preview is used for the ROI definition.

Clear ROI(s) before Definition

Any previously drawn stimulation ROIs (drawn by clicking Draw ROI(s) or during Runtime) are cleared if this option is checked.

Define Laser Powers

If checked, Laser Power Setting dialog window appears when drawing stimulation ROIs. This window defines the power of each laser line on each stimulation group. Each point from a MultiPoint experiment can carry a different definition of ROIs or laser lines and laser powers.

Note

To assign a stimulation ROI using DMD / Galvo XY to a preset, please register the preset using the DMD / Galvo XY Pad in advance.

ROIs >

Use ROI from Analysis

This task takes binaries created in analyses and applies them as stimulation ROIs during stimulation experiments.

Measure features of the cells using the cell count analysis and stimulate them later on in the job.

Requires:

Task:

Analysis > Define Analysis, Analysis > General Analysis, Analysis > Cell Count Analysis, Analysis > Intensity Analysis, Analysis > General Analysis RGB, Analysis > Wound Healing

Task Name

Name of this task.

Load from Analysis

Select the analysis task from which the binaries will be taken and used as stimulation ROIs.

Auto Focus

Defines auto focus on the selected ROI.

Stimulation

Lists the stimulation ROIs to which a stimulation configuration can be assigned using the ... button.

ROIs >

Assign ROI to Point/Well

This task assigns the stimulation ROI defined in the ROIs > Define ROI to a point of a point loop or a single well/row/column of a well loop.

Draw ROIS on a single well and assign them to different wells on a well plate which will be stimulated later on in the job.

Requires:

Task:

ROIs > Define ROI, Stage XY Points > Loop over Points or Well Plates > Loop over Wells

Assign ROI definition

Select the desired ROIs > Define ROI or ROIs > Use ROI from Analysis definition.

Choose to which element you want to assign the ROI.

ROIs🔗

Note

ROIs