CrEST

Crest_DeepSIM__AdjustReconstructionParameters, Crest_DeepSIM_CreateReconstructedImage, Crest_DeepSIM_CreateReconstructedImageND, Crest_DeepSIM_CreateRollingReconstructedImageND, Crest_DeepSIM_ExportRawChannels, Stg_SetDisk_On_Off, VCS_GetFocusLens, VCS_SetFocusLens

Crest_DeepSIM__AdjustReconstructionParameters

Description 

 Crest_DeepSIM__AdjustReconstructionParameters();

Adjusts the reconstruction parameters for the CrestOptics DeepSIM device.

Crest_DeepSIM_CreateReconstructedImage

Description 

 Crest_DeepSIM_CreateReconstructedImage();

Creates the reconstructed image acquired by the CrestOptics DeepSIM device.

Crest_DeepSIM_CreateReconstructedImageND

Description 

 Crest_DeepSIM_CreateReconstructedImageND();

Creates the reconstructed ND image acquired by the CrestOptics DeepSIM device.

Crest_DeepSIM_CreateRollingReconstructedImageND

Description 

 Crest_DeepSIM_CreateRollingReconstructedImageND();

Creates the rolling reconstructed no-delay time-lapse image acquired by the CrestOptics DeepSIM device.

When it is executed, it runs the reconstruction in sequence not only on the DeepSIM frames in each ND step, but also on the intermediate frames between them.

For example if the DeepSIM ND acquisition is run with DeepSIM High Throughput settings, meaning each DeepSIM frame consists of 17 camera frames. When the Rolling Reconstruction is executed, it works in the following way:

  1. Reconstruction on camera frames 1 - 17 (so all camera frames from DeepSIM ND image 1 as usual).

  2. Reconstruction on camera frames 2 - 18 (camera frames 2 - 17 from DeepSIM ND image 1, camera frame 18 from DeepSIM ND image 2).

  3. Reconstruction on camera frames 3 - 19 (camera frames 3 - 17 from DeepSIM ND image 1, camera frames 18, 19 from DeepSIM ND image 2) ....and so on over the whole ND.

    It is possible to run the Rolling Reconstruction only on No Delay Timelapses with just one DeepSIM channel, as it requires the images to follow one after another.

Crest_DeepSIM_ExportRawChannels

Description 

 Crest_DeepSIM_ExportRawChannels();

Exports the raw channels acquired by the CrestOptics DeepSIM device.

Stg_SetDisk_On_Off

Description 

 Stg_SetDisk_On_Off(
   int  DiskOn
);

This function turns on/off the disk rotation.

Parameters

int DiskOn

Is the disk rotation on?

0

Disk is off

1

Disk is on

VCS_SetFocusLens

Description 

 VCS_SetFocusLens(
   int  VCS_iPosition
);

Sets the VCS (VideoConfocal Super-resolution) focus lens position.

Parameters

int VCS_iPosition

Sets the VCS focus lens position.

VCS_GetFocusLens

Description 

 VCS_GetFocusLens(
   int *VCS_piPosition
);

Returns the VCS (VideoConfocal Super-resolution) focus lens position.

Parameters

int *VCS_piPosition

Pointer where the VCS focus lens position value is returned.