Displayed Thumbnails

Current Color Image

The currently active image.

Color Image for Undo

The last image saved automatically to the Undo History.

Reference Color Image

The reference image added by user via the the Reference menu. See Reference > Current Image -> Reference.

Current Binary Image

The currently active binary image.

Binary Image for Undo

The last binary image saved automatically to the Undo History.

Reference Binary Image, ROI Image, Reference Binary1 Image, Reference Binary2 Image

Commands from within the Reference menu were used to copy binary images to these positions. However, this functionality has been replaced by advanced commands which work with multiple binary layers. See Binary > Binary Operations, View > Analysis Controls > Binary Layers .

Current toolbar

Displays list of icons (command) in current toolbar.

Add

Adds a new toolbar command or a separator.

Remove

Removes currently selected command.

Up, Down arrows

Changes the order of icons on the toolbar.

Button properties

Displays icons in enabled and disabled state together with associated command.

Change

Press this button to change the image associated to command.

Command

Write command name (or name of more command), that should be associated to current toolbar button.

Paste

This submenu helps to insert commands into the command edit box. Choose whether to:

Paste Command

Opens the list of all available commands. Choose the one you would like to insert.

Paste Macro

Opens the Macro - Open dialog box in order to define a macro to be executed.

Paste History

Pastes the sequence of recently used commands in four steps:

1. Selecting commands from the command history.

2. Removing redundant commands - decide whether to remove doubled commands or not.

3. Adding, removing, or editing chosen commands.

4. Finishing the Paste History wizard.

Name

Displays name of current toolbar entry.

Tooltip

Displays tooltip, that will be displayed when you move over the toolbar button by mouse.

Acquisition

This panel shows basic acquisition tools (View > Acquisition Controls > Acquisition ).

Live

Displays the live camera image according to the camera settings (the button changes to Freeze when the live image is shown).

Capture

Acquires a single image from the camera.

Large Image

Opens the Scanning Wizard (Addons > Large Images > Scanning Wizard ) used for a step by step large image acquisition.

Save

Saves the current image.

Save As...

Opens a standard save as... dialog for saving image files.

Open

Displays a standard opening dialog for opening image files.

Light Paths

Shows available light paths (light tracks between the cameras and illumination devices) present in the current configuration.

Light Path

Click this button to open the Lightpath Scheme setting up the light paths.

Add

Opens the Calibration > New Optical Configuration dialog enabling to create a new optical configuration.

Cameras and Devices

Displays all cameras and devices included in the current light path. All camera and device controls are described in their particular section.

Cameras

Cameras

Illuminators

Illumination Devices

Other Devices

Other Devices

Browse for Folder...

The destination directory can be changed by clicking the Select Directory button in the combo box and search for a new folder. The setting is global for the File > Open/Save Next > Save Next and the Acquire > Auto Capture commands. List of recently used folders is displayed below the Browse for Folder... and Job Synchronization items.

Job Synchronization

This virtual folder automatically shows the images acquired by the last launched job.

# files

Number of images in the folder is indicated in the top left corner of the control window.

wrapping, thumbnail size

Image thumbnails can be lined up in a single row or in multiple rows using the wrapping buttons. Number of rows depends on the selected thumbnail size and on the size of the control window. Size of image thumbnails can be easily changed using the thumbnail size buttons.

Apply autocontrast to thumbnails

Press this button to apply LUTs autocontrast function to the displayed thumbnails.

Caution

Using this function, two almost-identical images may look different (brightness-wise). For example if the same scene is captured with and without a scale, the scale may cause the thumbnails to look differently.

...

Press the ... button and select the folder where images captured by the Capture and Store button will be saved. The path and file name of the next image to be saved is displayed on the left.

Burn Scale, Burn Annotations

Select which vector layers will be burned into the saved images. We recommend to use these options only if saving the captured images to a file format which is not capable of saving vector layers (bmp, png, ...). See also Supported File Formats. Note that mono images are automatically converted to RGB images after the scale/annotation is burned. Image data and quantitative information can be lost.

Live after Capture

Displays live image after the Capture and Store button is clicked.

Open after Capture

Select this option to open each captured image in NIS-Elements.

Capture and Store

Click this option and a new image will be captured and saved to the directory specified above.

Settings

Prefix, Next File, Digits

Type the prefix and numbering style intended for the files being saved automatically. The Next File field informs you and enables you to change the number which will be used for the next image saved automatically.

File Format, Compression

Select the file format to save the images in. Some formats enable you to set the Compression parameter. It is recommended to use either “none” or “lossless” in order to preserve good image quality.

Make Subdirectories Automatically

The images can be sorted to subdirectories automatically based on the time of acquisition. Select this option and use place-holders to specify format of the folder names (click to display a list of available place-holders). E.g.: “$YYYY$MM$DD” would create a new subdirectory for each day named like this: “20170103”.

Save to File

Check this item to save the image in a file. Specify the destination folder and name the file.

On camera format change

This option defines an action which should be done when camera format (resolution) has changed during the experiment. There are two possibilities: either a new image is created or the image is resized.

Capture on 'enter' key

Check this option to assign the Enter key to the Capture action.

Filter Name

Displays editable filter name.

Excitation/Mirror/Emission

Check which settings is used for defined filter.

Number of Bands

Specify number of used bands.

Type of spectrum

The spectrum can be defined by setting the from-to wavelength values, or by setting the peak value and the range of wavelengths. The number of text boxes depends on number of bands used.

Control Window Options

Show Groups

Press this button to sort the optical configurations displayed in the control window to groups. These groups can be expanded or collapsed by the +/- buttons next to the group name.

Regular buttons

After you press this button, all optical configurations button stretch to the same size.

New Group

Press this button to create a new group of optical configurations.

Explore Optical Configurations

Opens the Optical Configurations window. See Calibration > Optical Configurations for further description.

New Optical Configuration

Opens the New Optical Configuration window. See Calibration > New Optical Configuration for further description.

Lightpath Scheme

Opens the Lightpath Scheme pad showing the current microscope configuration with visible light paths.

Optical Configurations buttons

These buttons represent available optical configurations which are placed on the Optical Configuration toolbar. Press the corresponding button to select/unselect an optical configuration. Channel color for optical configuration is displayed next to each of the buttons. If you press the black arrow button, current camera and device settings will be assigned to the selected optical configuration.

Context Menu over Toolbar and Groups

New Group

Creates a new OC group with a given name.

Rename Group

Renames the group over which the user is inducing the menu.

Regular Buttons

Regular buttons can be turned on/off using this command.

Show Groups

Groups can be shown/hidden using this command.

Show Channel Color

Color next to each channel can be shown/hidden using this command.

Show Assign Button

Displays/hides the black arrow next to each OC button, used for assigning the current settings.

Show Main Toolbar

This command shows/hides the main toolbar containing control buttons.

Lock Window Geometry

This command can be used to lock the current size of the window. The window cannot be resized until the geometry is unlocked again.

Show Optical Configurations Toolbar

All OC buttons can be shown in the main toolbar using this command.

Font and Color

Opens the Properties dialog where the font type, font size, font style, and text and background colors of the OC buttons can be changed for the current group.

Context Menu over OC buttons

Assign current objective

Assigns the current objective to the selected OC.

Assign current camera setting

Assigns the current camera setting to the selected OC.

Assign current microscope setting

Assigns the current microscope setting to the selected OC.

Button Properties...

Opens the Properties dialog window enabling to adjust the type, size, style and color of the font including the background color of the selected OC button.

OC Panel

Reveals a context menu described above.

Calibrate using Objective

Calibrates the selected OC using the current objective.

Select/Unselect

Selects/deselects the OC button.

Copy to

Copies the settings of the current OC to the OC chosen from the context menu.

Duplicate

Copies the selected OC into a new OC with a given name.

New...

Opens the New Optical Configuration dialog window enabling to define a new OC which is then added to the OC Panel.

Remove...

Removes the selected OC from the OC Panel.

Rename...

Renames the selected OC.

Edit...

Opens the Optical Configurations dialog window enabling to edit the selected OC.

Initialize

This button initializes the slide loader and retrieves information about slides and cassettes present in the device.

Scan Cassetes

Starts scanning of slides of all cassettes present in the loader.

Scan Barcodes

Scans bar codes of all slides.

Stage

Indicates the current stage state (empty or loaded).

Unload

Removes the slide currently loaded on the microscope stage and returns it to its original position.

Stop

If some unexpected event occurs or a crash is at hand, press this button to stop the slide loader immediately.

Relative Movements

XY

Coarse, Fine

Two accuracy settings of movement can be set to Coarse or Fine. Whenever an arrow button is clicked, the stage moves by one step in the selected direction. Moving the stage in a diagonal direction, e.g. top-right direction, behaves like if the top and right moves are performed together. The current step size is displayed between the arrow buttons.

1. Click either the Coarse or Fine button which you want to define the step of.

2. Fill the desired value to the edit box in the middle. The value is set and remembered.

Note

Each physical device has its minimum step size. If the step value set in the edit box is smaller than this minimum, the device will move by the minimum achievable step without warning.

FOV

If you pressed the FOV button, the step size is calculated automatically in order to match the current camera field of view. The below pull-down menu reduces the calculated step size by the given percentage.

Z

Click the arrow to move the Z drive in the indicated direction with the predefined or custom step size. The arrow button with a sample () indicates the Z drive direction moving towards the sample.

Note

Some stages require Z calibration before Z navigation can be used. If your Z control is disabled (N/A), calibrate the stage using Devices > Calibrate Z.

Absolute Movements

You can move the stage(s) to any position by giving the system its absolute coordinates.

ESC

Moves Z drive to its escape position.

REF

Moves Z drive to the previously set refocused position.

Piezo

Available only when Piezo Z drive device is connected. Press the arrow button to open a submenu and select an action. This action is performed when you press the Piezo button. The Keeps Z position and centers Piezo Z option moves Piezo Z drive to the home position, but keeps the original position of absolute Z (sum of Z1 and Z2). The Move Piezo Z to Home position moves Piezo Z drive to the home position, regardless of Z drive position.

ND Acquisition Overview

Provides overview of the whole stage with the defined experimental points. It enables the user to modify, add and remove points, which are to be captured during the experiment.

Overview Elements

Points

Selected point is black. All other unselected points are green.

Line

A line marks path of the stage between two points.

Cross

A cross represents current position of the stage.

Area of Interest

Position of Area of Interest can be changed by pointing mouse cursor over the Area of Interest. Hold down the Ctrl key and the mouse cursor changes its shape to arrows with brown square. Now move the area to a different location. Edit borders of the area to change size or shape of Area of Interest. The status bar on the bottom of the window displays size if the Area of Interest.

Focus Surface

Provides overview of the surface used for focusing. It modifies, adds and removes the points, in which the system will refocus. Move the stage to at least 3 different XY positions, focus, and press the Add Point button each time. The points will determine a plane on which the system will always focus (anywhere on the specimen).

All points used in the focus surface can be redefined using autofocus. Being on the Focus Surface Tab, right-click the (xy) preview and select Auto Focus All Points. Auto focus will be performed and the Z positions modified.

Common Controls

Switch to Area of Interest

Press this button to hide the whole stage area and display only the Area of Interest.

Adjust to Points

Press this button to adjust view so all the points are displayed optimally.

Full size

Displays the whole stage overview in full range of the view.

Zoom In

Increases magnification of the view.

Zoom Out

Decreases magnification of the view.

Show Point Indexes

Press this button to display indexes of each point in the overview.

Show Scale

Press this button to display scale in the overview.

Show Point to Point Distance

Displays real distance between points in the overview.

Add Point

Adds a point at the current position of the stage. You can also use the space bar to add a point.

Remove Point

Removes selected point.

Remove All Points

Removes all points.

On Double Click Select Nearest Point

Double-clicking inside the XYZ Overview area moves the stage. If the button is pressed and you double click near the position where an experimental point is placed, the stage will move precisely to the coordinates defined in the experiment.

Go to First Point

Moves stage to the first set position.

Note

These commands move the stage to the set position if the Move Stage to Selected Point option is checked. Else the move is done only in the overview window.

Go to Previous Point

Moves stage to the previous set position.

Go to Next Point

Moves stage to the next set position.

Go to Last Point

Moves stage to the last set position.

Z Auto Scale

Automatically sets scale of the Z axis to provide the best view of the Z profile graph.

Show Focus Surface

Use this button to display Z coordinates of the focus plane in the Z profile graph.

Move Stage to Selected Point

Check this option to make the stage move to the selected point.

Special Controls - ND Acquisition Overview Tab

Show Scan Areas

Use this button to display scan areas. It displays area which matches to single captured image over corresponding point. This area depends on selected Objective or Calibration. If a scan area of one point overlaps some scan areas of different points, or scan area of current stage position (marked with a cross) - the color of those areas is red. If they do not overlap, then it is green.

Show Focus Surface

If the focus surface is defined, this button displays its color heat-map. The colors indicate whether the focus surface is tilted or curved. A perfectly horizontal focus surface would be displayed as a solid color.

Front View

Real position of the sample on the stage is shown in the overview (as seen when standing in front of the microscope).

Camera View

Stage overview displays the sample in orientation as it is shown in the live view. Rotation settings in Acquire > Camera Light Path are taken into account.

Show Z profile

Displays the Z profile graph in the bottom part of the window.

Leave PFS offset ON between points

Check this item to keep PFS on while moving between the points.

Special Controls - Focus Surface and PFS Surface tab

Redefine Z Position

Overwrites the currently selected point in the Focus Surface tab with the current Z position of the stage.

Reload

Each time NIS-Elements application is restarted, the focus surface points are cleared. If you want to use the points from the last session click on this button to reload the points.

Interpolation method

Choose the method used for point interpolation. Smooth and Nearest Neighbor methods are available.

Move Stage to Selected Point

If checked, the stage is moved to appropriate coordinates after a point is created.

Remove All Points

Deletes all PFS surface points.

Annotations

This toolbar enables you to insert vector objects to the image. The image itself is not affected by the content of the annotation layer, although annotations can be saved with it (JP2, TIFF, and ND2 formats can handle it). After inserting the selected object into the image you can select it using the Pointing Tool and right-click to edit its Properties.

• Insert Text

• Insert Arrow

• Insert Labeled Arrow

• Insert Line

• Insert Rectangle

• Insert Ellipse

• Insert Polyline

• Insert Polygon

Confirm with R-Click

If this button is active, each drawn annotation or measurement object has to be confirmed by the secondary mouse click. This is especially useful for adjusting the object's shape and precise placing.

Switch to Pointing tool after drawing new object

If turned on, the cursor is automatically switched to the Pointing Tool after drawing an annotation or measurement object.

Manual Measurement Tools

See the description of all available measurement tools in the Measurement Tools section.

Appending Labels

Text labels are automatically appended to every new measurement object if this button is turned on. Click the adjacent ... button to adjust visual properties of the label. The label format can be adjusted in the Manual Measurement section of the Measure > Options window.

Options

Press the Options button to display additional commands:

Measurement options...

Choose this command to display Measure > Options dialog window.

Load Configuration and Data...

Loads the previously saved measured data and configuration of their display from an external file.

Save Configuration and Data...

Saves the measured data and configuration of their display to an external file.

Use Class

Selects a class number from the combo box next to Options which is written into the Comment column of the results table. Use this feature e.g. with the Count tool to count cells (Class 1) and their nuclei (Class 2).

Set Class Count...

Defines the number of classes. Insert a numeric value.

Measurement Results

The measured values are being appended to the results table. There is one table for each measurement type. The measured data can be exported to an external file via the standard Export menu (see the Exporting Results chapter for further details).

Reset Data

Pressing this button will erase the currently displayed data, while the data from different types of measurement will not be affected.

Clear Screen

Removes all measurement objects from the image while the measurement data remain in the results table.

Statistics

Click this button to display an additional table where overall statistics (Mean, Standard Deviation, Minimum, Maximum, Sum) of the measured values are displayed.

Histogram

Optionally, histogram of the data inside the results table can be displayed by selecting the Show Histogram button.

Results Table Properties

Measurement results of the currently measured quantity is displayed in the table. Switching between measurement tools of different quantities automatically changes the table contents.

Note

The data remains in the table even after the application is restarted. The data will be erased only after the computer restart.

Contextual Menu Commands

Secondary mouse click in the results table reveals a context menu containing the following commands:

Show Full Path

If you have clicked on the Source column, this command enables you to display/hide a full path to the measured image file.

Hide Column, Show All Columns, Show Column

These commands enables you to display any combination of columns that is available for the particular measurement type.

Autosize Column

Selecting this command adjusts the column width according to the values it contains.

Delete Records, Select All, Invert Selection

These commands enable you to adjust the current records selection (made using Ctrl/Shift + mouse) and delete the selected records.

Sort By, Remove Sort

Enables you to select the column to sort the records by, and to remove sorting.

Feature of Interest

The measurement feature selected in this submenu is displayed in the histogram.

Units, Precision

Select units and precision for the display of figures.

Options

The Options button displays a window with options regarding the histogram appearance. Apart from common appearance settings like color, line width, or default text descriptions of the histogram items there are the following options in the window:

Mode

In the Mode pull-down menu, you can select the way of displaying the Y axis (number, number in %, cumulative, cumulative in %).

Line histogram

The histogram can be switched to be displayed as a continuous line instead of a number of bars.

Captions font

Choose a font and its size to be used for describing x and y axes of the histogram. Write your caption of the particular axis into the Caption edit box below.

Values font

Select a font and its size to be used when displaying numeral values inside the histogram.

Bins Definition

Select the Bins tab in order to adjust the way bins of the histogram are created. If the histogram bins shall be equidistant, bin width, minimum and maximum values can be set. If non-equidistant, each bin limit values should be put inside the definition table.

Graph/Table

You can switch between the graph and the table view via these buttons. The histogram source data are displayed in the table view.

Binary Toolbar

A simple set of tools for binary layers editing. See View > Analysis Controls > Binary Toolbar .

Thresholding

The most common tool for creating a binary layer. See Thresholding.

Pixel Classifier

A tool for classifying pixels. Since it generates a set of binary layers as well as the Thresholding tool does, both these tools are mutually exclusive (only one of them can be selected). See View > Analysis Controls > Pixel Classifier .

Binary Layers

For managing of binary layers in the image. See View > Analysis Controls > Binary Layers .

Restrictions

Restrictions applied to the measured binary layers. The purpose of this tool is to exclude the objects which do not match the given criteria from measurement. See View > Analysis Controls > Restrictions

Current field

This information displays the number of objects in current field.

Store Data

This button moves the Current data to the Stored repository.

Current/Stored

You can select which data repository appear in the table. The Current data are the currently measured data which can be yet modified by changing parameters in the Automated Measurement control window followed by clicking the Update Measurement button. The Stored data cannot be changed. Current data are appended to this repository each time the Store Data button is pressed, or if the Measure > Perform Measurement command is run.

Measurement Method

This pull-down menu selects the type of measured data. The selected method updates the entire table and the Graph (histogram) area.

Object Data

Each row of the table represents object features for a single object.

Object Stats

Table data show mean feature values calculated from all objects present in the selected frame and the selected binary layer.

Field Meas.

Lists one field (frame) per table row containing the selected field features.

ROI Data

Lists one ROI per table row each containing statistics of all objects (from all binary layers) inside/touching the particular ROI.

Binary in ROI

Lists one ROI and binary layer per table row each containing statistics of all objects (from a given binary layer) inside/touching the particular ROI.

Reset Data

This button clears all data.

Clear Current Measurement

Press this button to clear the current measurement.

Show Statistics

Press this button to show the statistics.

Show Histogram

Press this button to show the histogram.

Time Series Graph

Press this button to display the Time Series Graph.

Show Object Catalog

Press this button to display the View > Analysis Controls > Object Catalog window.

Export

Exports the measured data to various locations. See Exporting Results for more information.

Options

Press the Options button to display additional commands:

Select Object Features

Choose which features are measured. See Measurement Features. You can also select the Comment feature which enables user to insert arbitrary comments to measured items in the data area. The length of the comment is limited by 63 characters.

Note

Automatic updating features update the whole result table. If the Keep Updating or the Update Measurement feature is used, the content of the Comment column will be removed automatically.

Select Field and ROI Features

Choose which features are measured. See Measurement Features. You can select the Comment feature, too (see above).

Measurement options

Choose this command to display Measure > Options dialog window.

Load Configuration and Data

Loads the previously saved measured data and configuration of their display from an external file.

Save Configuration and Data

Saves the measured data and configuration of their display to an external file.

Caution

This command does not save the Current data. To make sure, all data will be saved to a file, click the Store Data button beforehand.

Update Measurement

Press this button to update the current data. Once measurement has been performed, its parameters can be still modified within the View > Analysis Controls > Automated Measurement panel. This button must be pressed in order for the changes to take effect on the data.

Keep Updating

Press this button to keep the data continuously updated.

Data Area

Grouping

You can arrange the view of images efficiently by grouping them. You can group images according to any feature, or comment. Drag the column name bar to the grouping bar (right above the column name bars). All files with matching field values of the selected column will be grouped together. This can be undone by dragging the column caption back to the others. See Figure 153, “Organizer layout” (the Dimensions column is grouped).

Data window

The features are gathered in columns. Click the column caption to sort the data ascending or descending.

Histogram/Data Table

The right portion of the window contains data analysis and visualization tools. You can display the histogram of the values or a statistics by pressing the adjacent button. Choose the measurement feature from the pull down menu and its distribution is displayed in the histogram. The data are processed. You can export them to a report, MS Excel application or clipboard. Histogram properties can be changed via the Options button (see: Options). If you have grouped the data, you can also display the histogram of a subgroup. The group can be selected from the Groups menu.

Contextual Menu Commands

Select Field and ROI Features

Opens a window with a list of measurement features. Select which features you want to measure and press the Add button.

Hide/Show/Autosize Columns

Hides the column you are currently pointing at. To make the column visible again, select it in the Show Column pull down list.

Default Order

Orders columns by default.

Delete selected records

Removes selected entry.

Mark as Invalid

Marks selected entry as invalid data.

Purge Invalid Records

Purges records marked as invalid.

Select All/Invert Selection

Enables the user to select or invert selection of the data.

Sort by

The data can be sorted according to selected column title in descending or ascending order.

Feature of Interest

Select a feature you want to inspect in more detail. A histogram (or statistics) of the feature values appears in the right portion of the control window.

Units

Displays the list of all possible units. Select the unit you want to use.

Precision

Select the precision of the digits.

Contextual Menu Commands

When you right click a thumbnail or binary layer name, a contextual menu appears with additional commands:

Select All Layers

This command selects all binary layers.

Copy Selected Layers

This command copies selected binary layer to clipboard.

Paste Layers

This command pastes copied binary layer.

Layer name

Name of currently active binary layer is displayed.

Remove

This command removes selected binary layer.

Rename

This command renames selected binary layer.

Duplicate

This command duplicates selected binary layer.

Clear

This command clears selected binary layer from document.

Layer Color

Color of the binary layer can be changed from this color pull down menu.

By Object

Uses a smart algorithm to prevent neighboring objects to have similar color.

By Object ID

Colorizes objects by 12 different colors in the direction from the top left corner to the bottom right corner.

By Object Value

It is possible to colorize objects according to a measured value.

Attach to Component

This command opens list of components to which the selected binary layer can be attached.

Detach Component

This command detaches selected binary layer from the component back to all components of the document.

Common Tools

Pointing Tool

Basic tool for selecting objects in the image.

Results by Reference

If this button is pushed, only the results linked to the selected reference object are shown. If the button is not pushed, all reference measurements linked to all reference objects are shown.

Settings (meas, ref, dim, Points of Interest)

These drop-down menus are used to change the color and size of the measurement objects, reference objects and dimension lines. In the References and Stars tab, points of interest can be further specified.

Confirm on Right Click

Confirms the drawing of a measurement object by the secondary mouse click.

Switch to Pointing Tool After Drawing

Automatically switches to the pointing tool after drawing a measurement object.

Show/Hide Statistics

Shows/hides the statistics area at the bottom of the measurement table.

Show/Hide Labels

Shows/hides the measurement labels.

Columns Visibility...

Opens the Columns Visibility dialog where it is possible to select which measurement features are shown as columns. The same dialog can be opened from the context menu over the results header.

Show Results from All Documents/Show Results from the Current Document

Displays the measurement results either from all images or just the current image.

Delete Measurement

Deletes all measurements from the currently selected tab.

Delete All Measurements

Deletes all measurements from all the measurement tabs.

Export to Excel

Drop-down menu next to this button selects whether to export the measurement data and statistics to Excel, to a File or to Clipboard and whether all measurement tabs are exported as separate excel tabs (All Tabs) or just All Visible Columns are exported. Once the selection from the drop-down menu is made, click this button to perform the actual export.

Units

Defines the measurement units.

General

2 Points

Draws a line defined by two points (shown as crosses).

Simple Line

Draws a simple line.

Vertical Lines

Draws two parallel vertical lines.

Horizontal Lines

Draws two parallel horizontal lines.

Free Parallel Lines

Draws two parallel lines with a free rotation. Draw the first line, confirm it by a secondary mouse click and repeat this procedure for the second line.

Crosses

Draws a line defined by two points which are highlighted by crosses over the whole image.

3 Points Arc

Draws an arc by clicking 3 points on it. The second point defines the direction and the third point defines the diameter dynamically during its placing.

3 Points Circle

Draws a circle by clicking 3 points on it.

Circle

Draws a circle by clicking and dragging.

Autodetect Circle

Automatically detects a circle with its center in the center of gravity of the clicked object (confirm its detection by the secondary mouse click). The circle has the same area as the detected object.

2 Points with XYZ Stage

This function is used for measuring the distance between two points going outside the field of view. Run the live image, click a point in the image, move your stage and click in the image again. The line between the two clicked points is measured.

Crosses with XYZ Stage

This function is used for measuring the distance outside the field of view with auxiliary crosses. Run the live image, move the first cross to your point of interest and confirm its position by the secondary mouse click. Move your stage and repeat the procedure for the second point. The distance between the two points is measured.

Note

If an XY/Z stage is connected, coordinates of its current position are shown on the right side of the Distance Measurement panel.

Segments

Define Segments by Polyline

Draw a polyline by clicking into the image and confirm it by the secondary mouse click. The segments defined by the nodes are automatically separated and numbered in the results table. Secondary-mouse click on the header of the measured feature and select Show ... on Label to label each segment in the image with its measured value.

References

To measure reference distances, a reference object has to be defined first. Start with a reference tool from the Distance To toolbox. If multiple reference objects are defined, select one from the drop-down menu next to the reference tools. Then choose which distance is to be measured from the measurement tools on the left.

Point Distance

Measures the distance between the reference object and this defined point.

2 Point Line Distance

Draws a line with two points. The orthogonal distance between this line and the reference object is measured.

Simple Line Distance

Draws a simple line. The orthogonal distance between this line and the reference object is measured.

Linear Pitch

Measures the distance between two drawn parallel lines which are orthogonal to the reference line.

Linear Continuous Pitch

Measures the distances between multiple clicked parallel lines which are orthogonal to the reference line.

3 Points Circle Distance

Draws a circle by 3 points and measures the distance between the circle center and the reference object.

Auto Detect - Circle Distance

Automatically detects a circle with its center in the center of gravity of the clicked object (confirm its detection by the secondary mouse click). The circle has the same area as the detected object. Distance and angle between the circle center and the reference object is measured.

Concentric Circle

Draws a concentric circle with the reference object as its center and measures the distance features between them.

Circular Arch

Draws two points which define the arc on the reference circle which is measured.

Circular Continuous Arch

Draws multiple points which define the arch segments on the reference circle which are measured.

Define Reference Point

Defines the reference as a single point.

Define Reference Line

Defines the reference as a simple line.

Define Reference Circle

Defines the reference as a circle set by three points.

Define Reference Circle - Auto Detect

Defines the reference as a circle detected automatically around the selected object. Click on a visible object and confirm its detection by the secondary mouse click.

Stars

This type of measurement is used to measure reference distances and angles of objects around one reference object which has to be defined first. Start with a reference tool in the Distance To toolbox. If multiple reference objects are defined, select one from the drop-down menu next to the reference tools and choose which distance is to be measured from the measurement tools on the left.

Point Distance

Measures the distance and angle between the reference object and this defined point.

3 Points Circle Distance

Draws a circle by 3 points and measures the distance and angle between the circle center and the reference object.

Auto Detect - Circle Distance

Automatically detects a circle with its center in the center of gravity of the clicked object (confirm its detection by the secondary mouse click). The circle has the same area as the detected object. Distance and angle between the circle center and the reference object is measured.

Concentric Circle

Draws a concentric circle with the reference object as its center and measures the distance features between them.

More measurement features can be added from the context menu over the table header (Columns Visibility), such as Cref-C (distance between the center of the reference circle and the center of the second circle) or Cref-E (distance between the center of the reference circle and the edge of the second circle).

Define Reference Point

Defines the reference as a single point.

Define Reference Circle

Defines the reference as a circle set by three points.

Define Reference Circle - Auto Detect

Defines the reference as a circle detected automatically around the selected object. Click on a visible object and confirm its detection by the secondary mouse click.

Circles

3 Points Circle Distance

Draws a circle by 3 points and measures the distance and angle between the circle center and the reference object.

Auto Detect - Circle Distance

Automatically detects a circle with its center in the center of gravity of the clicked object (confirm its detection by the secondary mouse click). The circle has the same area as the detected object. Distance and angle between the circle center and the reference object is measured.

Concentric Circle

Draws a concentric circle with the reference object as its center and measures the distance features between them.

Define Reference Circle

Defines the reference as a circle set by three points.

Define Reference Circle - Auto Detect

Defines the reference as a circle detected automatically around the selected object. Click on a visible object and confirm its detection by the secondary mouse click.

Angles

Angle

Draws two connected lines and the angle between them is measured.

Angle between Lines

Draws two separated lines and the angle between them is measured.

3 Points Angle

Draws two lines defined by clicking three points and measures the angle between them.

4 Points Angle

Draws two lines defined by clicking four points and measures the angle between them.

Angle To Reference Line

Draws a line and measures the angle between this line and the previously drawn Reference Line.

Define Reference Line

Defines the reference as a simple line. Set the reference line from the drop-down menu.

Set Angle Range

Sets a range in which the angles are measured.

To display the Z Profile

• Click the View > Analysis Controls > EDF Z-Profile command to display the control window

• Open an ND2 file containing the Z dimension.

• Use the Applications > EDF > Create Focused Image command.

• The graph appears in the control window automatically.

Figure 577. Z-Profile Graph using two profile lines

To add/remove profile lines

• To add more profile lines to the image, use the context menu over a profile line and select Add Z-Profile Line.

• To remove an existing line, use the context menu over a profile line and select Remove Z-Profile Line.

To change the profile line color/width/style

• To change the appearance of a profile line, use the context menu over the line and select Line Properties....

Graph/Data/Measurement Tabs

There are three tabs at the bottom of the Z Profile control window named Graph, Data, and Measurement. Switch from the default Graph tab to the Data tab. The number, X, Y, and Z coordinates of every point of the Z profile is displayed there in a table. They can be exported using the Export button described below.

Some measurement actions can be performed within the graph. The results are written to the Measurement tab.

Tools

Hide/Show profile

This button displays/hides the profile line inside the image window. The profile line can be dragged by mouse and the length can be modified. In the 3D Surface view mode (click Show EDF 3D Surface View button on the image toolbar) the EDF Z-Profile plane can be moved by clicking Ctrl and dragging with the left mouse button.

Z-Profile Options

The appearance and behaviour of the graph can be modified in the Z-Profile Options window. Press this button to display it or click the General Profile Properties... from the context menu over a profile line.

Export

Click this button to display a pull-down menu. The Graph image, graph data, and the measurement results can be exported:

To MS Excel

The data and measurement tables can be exported to MS Excel. A new XLS sheet opens and the table is copied to it automatically. There is also the Export All To Excel option, which copies the data table, measurement table, and the graph image into it.

To a file

The data and measurement tables can be exported to an external *.txt file, the graph image to a *.bmp file. Select the command from the pull-down menu and define the target file name in a standard Save As-like window, which opens. Confirm the export by the Save button.

To clipboard

The data table, measurement table, and the graph image can be exported (copied) to Windows clipboard. Then the data or the image can be inserted into any appropriate application (text editor, spreadsheet processor, graphics editor) typically by the Paste command.

To report

The Graph image can be exported into the Report Generator (see: Creating Reports).

Measurement on Graph

The following interactive measurements can be performed within the graph. Please see the details in the Measurement on Graph chapter.

Note

Be aware that the scale in the X axis and the Y axis directions usually differ. In such case, the measured value does not match the angle displayed on the screen.

Distance from Baseline

Use this function to draw a red reference line (base line) by clicking and dragging in the graph area. Then click any other point(s) in the graph to measure its orthogonal distance to the base line. Finish the measurement by the secondary mouse click.

Clear Measurement Objects

Deletes all the measured objects in the graph.

Reset Data

Clears the table with the measurement results.

Show Measurements

Use this button to show a table containing results of measurements made in the graph.

Shift Profile Line

Enter the shifting step into the edit box (units are taken from the current image) and click on one of the arrows to move the profile line in the X axis.

Show 1:1 Axis Ratio

Sets the axis ratio of the graph to 1:1 so that both axes have the same calibration.

Zooming

The Z-profile graph may be zoomed in the usual way. You can click the zoom buttons placed on the control window sides (please see the Image Window chapter for the buttons description) or a mouse wheel can be used:

Standard zoom

Point to the target area and roll the mouse wheel. The graph will be zoomed in/out according to mouse wheel movement direction.

Horizontal axis zoom

Zoom while holding the Ctrl key down.

Vertical axis zoom

Zoom while holding the Shift key down.

General Options tab

Figure 578. Z-Profile Options dialog window.

Graph

Choose background, axes and grid colors from the palette. Check the Graph Title option to display a title, whose text you can enter in the adjacent field. Then check the Show Horizontal/Vertical Grid items if you want to make them visible in the graph. If X/Y Axis Always Visible item is checked, the axes do not leave the graph area while zooming in the graph.

Data Lines

Select interpolation method or drawing the graph lines by pressing the relevant button. You can choose Steps (rough), Linear (smoother), or Bicubic (really smooth) interpolation method.

Graph points

Check this item to display data points. Small dots indicating the actual data values position can be displayed on the graph line. The points appear only if the distance between them is big enough for them to be recognized (they usually appear when you zoom in the graph).

Anti Alias

Turning this option ON will make the graph lines look smooth.

Fill Graph Area

Check this item to fill in the area under the line chart with a color. Select the amount of opacity used from the list of predefined values.

Profile line

Defines the color and width of the profile line.

Delete previous measurement from graph automatically

If this option is checked, the new graph measurement always overwrites the old one. If the function is not checked, measurement history remains in the graph.

X axis

Figure 579. 

Graph Range

The Auto option enables that the range values are set automatically. On the contrary the range values can be set on Fixed values also. The range values can be set for minimum of X axis, maximum of X axis, major and minor grid of X axis.

Zoom

If you select the Best Fit option, zoom of the X axis will be set to best fit. Otherwise you can set the Fixed range.

Y axis left

Figure 580. 

The Graph Range and Zoom options are described above.

Left Data line pen

Set the line appearance - line color (in case color of image component is not set), thickness and style (solid, dot, dashed, dash-dot).

Top Toolbar

Fill Object Catalog

Press this button to fill the Object Catalog with data from Automated Measurement Results.

Data Source

Choose the data source. Either the Object Count or the Automated Measurement data source is automatically set according to from which window was the Object Catalog invoked.

Object Catalog Options

Press this button to change the Object Catalog Settings.

Automatic Orientation Mode

Press this button to adjust automatically orientation of all objects.

Sort

Select the sorting features (Feature of Interest, Ascending or Descending sort) from the pull down menu that appears after you press this item. The Feature of Interest item is available only for option Unsorted. It displays by which feature the objects are sorted.

Define Filter

Press this button to define the filter. This button opens the Define Filter window.

Figure 582. 

Select a feature from the list of relevant features in the pull down menu and set the range values.

Note

When using the results from Object Count, the filtering options in Object Catalog are unavailable.

Filter

Press this button to apply the filter set in the Define Filter window. To discard the filtering conditions, press this button once again. When filtering is active, the icon is highlighted red.

Note

When using the results from Object Count, the filtering options in Object Catalog are unavailable.

Viewing

Edit the style of viewing the thumbnails.

Equal Zoom for All Objects

Check this option to set the same zoom for all thumbnails.

Thumbnail Size

Choose the thumbnail size: Icons, Small, Medium, or Large.

Snapshot

Press this button to create a snapshot of all object in Object Catalog. A new image is created.

Update Measurement

Press this button to update object catalog.

Contextual Menu

Delete Selected Records

Deletes selected objects.

Delete All Records

Deletes all records in the Object Catalog.

Mark as Invalid/Valid

Invalidates/validates selected objects.

Purge Invalid Records

Deletes invalid objects.

Move stage to measurement position

Move stage to object position.

Object Catalog Options

This window displays the Object Catalog setting.

Figure 583. 

Thumbnail Cutting Options

Bounding rectangle with border

Choose this option to let the thumbnails size reflect the size of objects. Define size of the border in micrometers.

Fixed rectangle

Choose this option to display the thumbnails in rectangles with fixed size. Define the rectangle dimensions in micrometers.

Objects

Hide image data around object

Check this option to hide surroundings of the objects.

Draw contour

Check this option to draw the contours of object.

Draw binary overlay

Check this option to display the binary overlay in the thumbnail image.

Generate new overlay color for each field

If you check this option, the objects will have unique overlay color for each measured field.

Show feature values

Check this option to show the feature value of object in left top corner of thumbnail.

Equal zoom for all objects

This option makes the objects in the catalog comparable by size.

Grid

Regular square grid

Check this option to show the thumbnails in regular square grid.

Draw grid lines

Check this option to draw grid lines between thumbnails.

Control Window Options - the Training Mode

Define Classifier

This button switches between the standard and the training mode of the classifier. Click Define to start training the classifier.

Pick samples from current image

These buttons are available only in the training mode. Use one of these buttons , , and click into the image to select pixel(s) which will be used as samples for the current class.

Reset samples

Press this button to delete all samples from the current class.

Undo/Redo

Standard Undo and Redo commands.

Smooth

This command applies smooth operation on the classified objects. Choose the smooth strength from the pull-down menu next to the button.

Show Scattergram

This button displays the scattergram.

Keep Updating Classifier

Press this button to run the classification continuously. Any changes in the image influencing the classification are shown immediately.

Classify in ROI On/Off

Press this button to determine if pixel classification is done only in ROIs (Classify in ROI On) or in the whole image (Classify in ROI Off).

Classification Method

Select a classification method of evaluating the binary objects. Manual enables defining the classes manually. If Bayes is selected, classes are defined via an algorithm for calculating classes from the defined samples.

Data selection

Select which data are used for classification: Intensity; Channels; Hue, Saturation, Intensity (for RGB images); or Ratio from the picker. Then specify further details picking the appropriate item from the Channels menu.

Classes

This window contains list of all defined classes. When in the training mode, you can change the name or the displayed color of each class. If you check the Background option, the selected class is set as background. The Area values define size of the selected class area in proportion to the whole image and also in document units. Press the Test button to display classified objects in the image. Check the Show All option to display all classes in the image. If you do not check this option, only the currently selected class is displayed.

Histogram

The slider on the left side defines scale of the histogram in the definition area. To define classes, drag the borders between them or set the value manually in the field which appears when you move your mouse cursor near the border line.

Control Window Options - Standard Mode

Update Classification

Runs classification on the current image.

Classify ND image

Runs classification on the whole ND2 file.

Store classified data

Saves current data from the classifier section into the stored data section.

Export

Exports the data to a file, MS Excel or clipboard.

Open / Save classifier

Stores and imports the classifier settings.

Data

This table contains all stored data.

Show Area Fraction button converts the stored data into the proportion of the total area [%].

You can use the Remove Data button to remove the current row or the Remove All Data button to remove all data present in the table.

Reset

Resets all restrictions to to default values.

Generate Binary Using Restrictions

Creates new binary layer using the set restrictions.

Select Object Features

Selects the measurement features. The Measure > Object Features dialog box appears.

Control Window Options

Show Groups

Press this button to sort the controls displayed in the control window to groups (Acquisition Controls, Analysis Controls, ...). These groups can be expanded or collapsed by the +/- buttons. Items in each group are displayed in alphabetical order.

Show Bitmaps

Press this button to display the controls represented by their icon only.

Show Text

Press this button to display the controls represented by their name only.

Customize

Press the Customize button to expand a menu containing all available control window sorted in four submenus. When you select any control from the submenu, the control is added to the control window overview portion and to the application top toolbar as well. The Add All Controls command adds all available controls to the overview and application toolbar at once. Similarly the Remove All Controls command removes all controls.

Associate with Current Layout

When this button is pushed, different contents of the control panel are saved for each layout.

Contextual Menu Items

Current/Reference Image

Use the three top commands to create a reference image, to load the reference image and to switch between the current and reference image.

Enable tooltips

When you place the cursor over the image preview, a tooltip containing information about name, filename, path, dimensions, size, and calibration is displayed.

Show scale

A scale can be displayed in the preview image. Its length is estimated automatically in order to be readable and depends on the image size. The units used depend on the image calibration.

Rescaling method

Choose the rescaling method: linear or quick.

Show reference image

Displays the reference image in the window.

Close

This command closes the opened image.

Image Properties

This command displays a dialog-window where all meta-information of the current image is shown. The dialog differs according to type of the image. All records are editable, some of them have predefined possible values. For more information see File > Image Properties.

State Indication

The arrows indicates the motion. The Last move statement indicates that Z device has stopped moving. The arrow orientation indicates the direction of the motion. The Moving statement indicates that the Z device is moving up or down (again the arrow indicates the direction).

Settings

Opens the Settings dialog which can be used to select basic system information which is displayed in the list of the System Information window.

Figure 591. 

Reversed Z Direction Indication

Check this item to reverse the indication of Z moving.

Select All

Selects/deselects all items in the list.

Show/Hide Graph

Displays/hides the graph view.

Select Graph Variables

Opens a dialog enabling to select which variables are shown inside the graph.

Run

Starts to monitor selected variables and displays them in the Graph.

Pause

Pauses the variable monitoring.

Reset

Resets the graph view.

Export

Once the graph area is paused this function exports data/graph into MS Excel, clipboard or into a file.

Differences only

If turned on, only changes of the variable are shown inside the graph (time is ignored).

Control Window Options

Regular Buttons

Use this command to change size of the buttons to be the same width. When this button is not pressed, buttons wrap the title (a text or an icon) of the button.

Add Command

This command opens the Paste Command dialog. You can choose a command, which is added to the Macro Panel.

Add Macro

This command opens the Add Macro dialog. You can select a predefined, shared or recently used macro or a macro from a file, which is added to the Macro Panel.

Setup Macro Panel

Opens the Setup Macro Panel window. You can manage the macros and commands and their properties.

Associate with Current Layout

When this button is pushed, different contents of the control panel are saved for each layout.

Setup Macro Panel Options

Figure 594. 

Overview of added macros and commands is displayed in this window. Name of the command or full path to the macro is visible in the first column. The second column shows names of the commands/macros. The third column displays assigned hot keys. The forth column contains preview of the button in the Macro Panel. You can directly edit the name, hot key or preview, if you left-click the corresponding field of the selected command/macro.

Add Command

Opens the Paste Command dialog.

Add Macro

Opens the Add Macro dialog.

Edit

Opens the Macro Editor window.

Properties

Opens the Properties dialog.

Move one line up/down

Moves the selected macro or command one line up/down.

Remove

Removes selected command or macro.

Remove All

Removes all commands and macros.

Load

Loads the saved definition from an external XML file.

Save

Saves the definition to an external XML file.

Globals/Locals

This tab displays all user defined variables of the currently running macro and their values. If no macro has been run, the table is empty.

System

There are some variables predefined within NIS-Elements. Their current values are displayed in this table.

Constants

System constants are listed within this tab.

Watch

A selection of local and global variables can be displayed within this tab. Write a variable name to the Name field, or if you right click the variable name within the Locals/Globals tabs, a context menu appears with the Send to watch option to be selected.

New

Creates a new LUTs gradient. Enter the name of your gradient.

Remove

Removes the selected LUTs gradient.

Duplicate

Duplicates the selected LUTs gradient. New name needs to be filled in.

Save current

Saves the gradient to an external XML file.

Load

Loads a previously saved gradient.

Up / Down arrows

Clicking the arrow button moves the selected gradient one line up / down in the list.

Name

Edit the name of your gradient in this field.

Interpolate colors

Check this item to make smooth color transitions between defined colors.

Absolute values

When checked, the gradient uses only discrete pixel intensity values with no color transitions between defined colors.

Symmetric values (center in 50%)

Defined colors are stretched symmetrically across the whole intensity range with 50% in the middle.

Intensity

Define the range of pixel intensity (in percentage), which corresponds to the appropriate Color.

Use this color picker for selecting the pixel intensity value directly from the image.

Color

Shows the chosen color. Press the ... button to choose an arbitrary color.

Reset Pseudocolors

Use this button to reset the currently selected colors to a default gradient.

Help

Displays the relevant help page.

Context menu over a scale

Burn Scale

Inserts the current scale layer into the image so that the scale becomes part of the image.

Default Scale Position

Moves the scale bar into its default position.

Lock Scale Position

Locks/unlocks the scale bar in its position.

Scale Properties

See View > Layers Properties > Scale Properties.

Line color

Sets color of the boundary line.

Line width

Sets thickness of the boundary line.

Position X

Sets the position of x-coordinate of the top left corner of the probe rectangle.

Position Y

Sets the position of y-coordinate of the top left corner of the probe rectangle.

Width

Sets width of the probe.

Height

Sets height of the probe.

Keep in view

If checked, the probe remains always visible while zooming and scrolling the image.

Type

Sets the type of displayed graticules:

• Rectangular Grid

• Circle

• Simple Circle

• Cross

• Industrial Cross

• Simple Cross

• Vertical Ruler

• Horizontal Ruler

• Graticule Mask

Line colors

Most grid types consist of primary and secondary lines. Their color can be selected from these pull-down menus.

Line width

The thickness of graticule lines can be selected.

Density, Automatically adjust density

The density value - the closest distance between two line intersections of a graticule - can be set in the edit box, or you can let NIS-Elements adjust it automatically according to the current zoom factor. The units selection depends on the image calibration (calibrated/uncalibrated).

Secondary grid

Adds vertical lines to the grid.

Scale Tab Options

Orientation

Sets the horizontal or vertical orientation of the scale.

Type

Sets the shape of the scale.

Size

Sets the size of the scale. If the Automatically adjust size option is ON, the Size edit box becomes disabled and the system computes the optimal size according to the image calibration and zoom factor.

Line color

Sets the scale bar color.

Line width

Sets the thickness of the scale bar line.

Background

Sets the scale background color. This is handy if your image has lots of colors and it is difficult to choose a contrasting color for the scale.

Keep in view

This option dynamically places the scale so that it is always visible, even if you zoom in or shift the image.

Show Text

This option hides/displays the scale legend.

Automatically adjust size

Check this item to enable the automatic scale size adjustment based on the image calibration and zoom factor.

Relative Size

Size relative to the image width can be selected here. To enable this function check the above option first.

Burn Scale Method

This option determines how the inserted scale will be resized - it can prevent inserting a too small or too large scale. Original burns the scale in the size as you see it in the image in the current zoom. Fit to Screen burns the scale as if you zoomed the image to the Fit to Screen mode and applied the Original method. 1:1 burns the scale as if you zoomed the image to 100% and applied the Original method.

Font Tab Options

Font

Standard font properties of the scale legend can be adjusted within the Font tab:

• Font type

• Size

• Alignment

• Vertical Alignment

• Style (Bold, Italic, Underlined)

Line color

Sets the frame line color.

Line width

Sets the frame line thickness.

Position X, Y

Sets the frame left corner coordinates.

Width, Height

Sets the frame dimensions.

Units

You can select the units of the measurement frame. Pixels and the calibration units are available in case of a calibrated image.

General Options tab

Figure 602. 

Graph

Choose background, axes and grid colors from the palette. Then check the Show ... Grid items if you want to make them visible in the graph. Check the relevant .. Axis Always Visible item to make the axis always visible while zooming in the graph.

Data Lines

Select interpolation method for data treatment by pressing the relevant button. You can choose Steps, Linear, or Bicubic interpolation method.

Logarithmic

Check to use logarithmic scale in the graph.

Graph points

Check this item to display data points. Small dots indicating the actual data values position can be displayed on the graph line. The points appear only if the distance between them is big enough for them to be recognized (so they usually appear when you zoom in the graph).

Anti Alias

Turning this option on will make the graph line edges look smooth.

Fill Graph Area

Check this item to fill in the area under the line chart with a color. Select the amount of opacity used from the list of predefined values.

Show point coordinates

Check this item to display or hide X, Y coordinates of pixels in the Data tab of the Intensity Profile control window.

Second line color highlight

If two profile lines are used, this feature highlights the second line inside the graph view.

Profile line settings

Set the profile line appearance. You can change its color, width and style, line thickness.

Note

These settings are applied to the profile line displayed over the image as well as to the graph lines, with only one exception: If image channels have their color specified (e.g. RGB image), the graph lines are displayed in this color and the color specified within this window is ignored.

Neighborhood

By defining a neighborhood, you extend the source area from 1pixel line to a stripe. Specify the Width of the stripe and its units - in (pixels, microns) and select which value to display within the graph:

• Mean - mean intensity of the stripe width is displayed in the graph

• Max - maximum intensity of the stripe width is displayed in the graph

Check Display in the image to visually indicate the neighborhood in the image.

Note

If a custom value, which is not listed in the Width box, is entered, the neighborhood measurement may be performed in an area narrower than specified due to pixel truncation.

Graph Measurement

Define colors of lines and labels used in the graph when objects are measured.

Indicator

When you click into the graph area, a vertical line appears in the graph and the “Indicator” appears in the image as well. Indicator is a line which displays the position on the profile line corresponding to the mouse position in the graph. You can select its color within this pull down menu.

Line Shift

Select pixels or the current calibration units for the Shift Left/Shift Right buttons in the tool bar.

X axis

Figure 603. 

Graph Range

The Auto option enables that the range values are set automatically. On the contrary the range values can be set on Fixed values also. The range values can be set for minimum of X axis, maximum of X axis, major and minor grid of X axis.

Zoom

If you select the Best Fit option, zoom of the X axis will be set to best fit. Otherwise you can set the Fixed range.

Y axis left

Figure 604. 

The Graph Range and Zoom options are described above.

Left Data line pen

Set the line appearance - line color (in case color of image component is not set), thickness and style (solid, dot, dashed, dash-dot).

Second profile pen

Set the second profile line appearance - thickness and style of the line (solid, dot, dashed,dash-dot). Check the Color highlighting to highlight the second profile line in the graph.

Zoom

Magnification of Magnifier Glass.

Size

Size of the Magnifier Glass.

Shape

Magnifier Glass can be either circular or square.

Border

Color and thickness of border.

Use gamma correction

The image displayed in Magnifier Glass can be enhanced by gamma correction in order to see the details brighter. The value for gamma correction is set in the box below.

Show magnifier window next to cursor

If checked, magnified image is shown next to the cursor whereas if unchecked, the magnified area is displayed around the center of the cursor.

Dialog Window Options

Stream Via

Internet - is used for streaming through an Internet service. In the Server drop-down menu, select a predefined Internet streaming service. Then click on the link below redirecting you to the website of the selected service. There you should register and acquire a streaming key. Enter this key into the Stream Key field. The button can be used to show the hidden characters.

Server - is used when the streaming server is installed on a different computer available on a network for NIS-Elements as well as for all clients. Enter the IP address into the Server edit box, check whether to access the stream with a password (Use Password) and click Register to register this copy of NIS-Elements for streaming on this server. To register this device on a different server, click Unregister. Click on the generated link to see the stream or use Copy Link and send the address to all clients which are about to watch the stream. To be able to use this option, Streaming Server has to be installed (contact your distributor to acquire the installation file).

Include Cursor

If turned on, cursor is included in the streaming video.

Start

Starts streaming.

Close

Closes the Stream Tool dialog window.