These workflows show preprocessing capabilities on whole dimension (many frames). They can be part of more complex workflows or used standalone.
Correlates the image contents frame-to-frame and translates each frame based on the calculated [x, y] offset.
Figure 678. Source Z-stack.
Figure 679. Aligned Z-stack.
Reduces a whole stack into a single focused image. It analyzes each frame and takes only portions of the image which are in-focus.
Figure 680. Source Z-stack.
Figure 681. EDF.
All mentioned operations reduce whole stack into a single image.
Figure 682. Source movie.
Note
Max IP is useful for showing moving objects and Median for static parts of the scene.
Select a previous frame with ND Processing & Conversions > Stack reduction > Select Frame and Image Operations > Arithmetics > Subtract it from the current frame. In the images below there is a plant root growing.
Figure 686. Source image Ii
Figure 687. Source Image Ii+1
Figure 688. Image Ii+1 - Ii
Figure 689. GA3 Schematic
Note
In many cases segmenting only what has changed in a scene may be much easier on a difference image like this one than on an original one.
