Inserts a single node with an overlay of all channels present in the current image.

Produces picture outputs representing channels of the source document:

Matching rules can be edited by the user for the correct mapping of channels from a different document. Matching of channels is done in following order:

Unmatched channels are assigned to the rest of the channels with the same channel type (no checking of wavelengths in this step). If there are no such channels available, missing channel is added.

It is possible to assign a custom description to each channel (e.g. nuclei, cell, spots).

Produces picture outputs representing both, the “All” channel composition and the individual channels.

Inserts a parental node with one channel selectable from the list of available channels (Selected) and the second channel with all the remaining channels together (Other).

Creates a new color image (channel) using the specified dimensions (Width, Height) and bit depth and fills it with the specified value.

Inserts a Deep SIM image.

Inserts a Deep SIM Z-Stack image.

Inserts all binary layers found in the source image and selected in the drop-down menu.

Inserts a parental node with one output containing the binary layer selectable from the list of available binary layers (Selected) and a second output with all the remaining binaries together (Other).

Inserts a binary node using a segmentation selected in the drop-down menu.

Inserts a ROI node using ROI(s) from the current image.

Inserts a table node showing the selected Result from the chosen Analysis and Module.

Loads the result table specified by the Result name from database and appends the content of the connected table to it. When the result table does not exist, the connected table is set as the result. It is useful when some analysis (statistics, classification) has to be calculated over all runs, not just the current one. In such a case use the result R to perform the analysis on.

Loads the result table from a HDF5 file given by the Filename parameter and appends records from node's input table. Table location inside the HDF5 file is defined by the current analysis name and node's output name.

Defines the folder used as an input. Click ... and select the folder.

Defines the input value.

Defines the input text.

Imports a color image from the specified path.

Imports a binary/Global ROI from the specified nd2 file.

Loads a binary layer consisting of rectangular objects representing bounding rectangles or centers of original binary objects. The main purpose of this node is the visualization of objects predicted by AI nodes. Optionally the objects' probability can be taken into account and objects can be filtered according to it.

The source table is assumed to be accumulated for the whole dimension and the first column represents the dimension index. The following columns are mandatory and represent the bounding rectangles' positions in pixels:

Imports a table from a comma separated values file (.csv). A data type (number or text) is determined for every column. The header (if present) can force the column type and a specific column meaning. Following header syntax is recognized:

Name or Keyword [Unit] : Type

Loads a table from HDF5 file.

Table can be:

When the format is not recognized table is not loaded.

Exports TIFF images to the specified folder with the given name. This action can be used for having a reference when GA3 is used more than once JOBS.

Figure 845. 

Exports objects from the connected binary layer into .svg images.

Creates new nd2 file from cropped regions of the original one represented as a multipoint loop. Frames are ordered according to the selected column from the input table. Other loops (Timelapse, ZStack) are preserved.

Exports the table into a .csv (Comma Separated Values) file.

Stores a table into a HDF5 file.

Exports the table into a .png image.

Loads data previously saved with the Sources & Reference > Temporary > Save Last Color node. The data is shared in the specified loop and kept private in other loops.

Saves the data to temporary storage. The data is shared in the specified loop and kept private in other loops.

Loads data previously saved with the Sources & Reference > Temporary > Save Last Binary node. The data is shared in the specified loop and kept private in other loops.

Saves the data to temporary storage. The data is shared in the specified loop and kept private in other loops.

Loads data previously saved with Sources & Reference > Temporary > Save Last Table node. The data is shared in the specified loop and kept private in other loops.

Saves the data to temporary storage. The data is shared in the specified loop and kept private in other loops.

Defines which channels (the connected ones) are shown in the wizard, whether autoscale LUTs is turned on and whether a pseudocolor is used for their visualization.

Defines which binary layers (the connected ones) are shown in the wizard, whether the object IDs are shown and how the binary layer transparency is set.

Defines which tables (the connected ones) are shown in the wizard.

Saves all connected channels into the result document. Connected inputs are synchronized by the “ToBeStored” property of the channel outputs present in the analysis.

Saves all connected binaries into the result document. Connected inputs are synchronized by the “ToBeStored” property of the binary outputs present in the analysis.

Saves all connected tables into the result document. Connected inputs are synchronized by the “ToBeStored” property of the table outputs present in the analysis.

Loads array components (25 components each) visible only in the preview. Cannot be stored.

Calls the reassign function and (modified) array components collapse back to mono channels visible in the preview. It can be stored.