I-SPT sets a maximal particle displacement or cut-of radius R for the linkage of two successive detections. I-SPT algorithm is a very restrictive tracking algorithm.
Figure 1619.
I-SPT provides a new tool to estimate the maximum particle displacement R and reduce tracking error. For further details on tracking error, check the advanced I-SPT section of this document Finding the maximum tracking displacement.
Select tab Displacement, then select Calculate Histogram. I-SPT suggests the maximum displacement which maximizes the number of correct detections : number of detections minus the error (green curve):
Figure 1620.
Put the value In the corresponding box
Figure 1621.
or use the button.
Click Calculate Histogram to compute another histogram.
Figure 1622.
Warning
The number of cut traces shall never exceed 10%. If it exceeds 10% tick Advanced Settings and change the parameters for detection (see Advanced I-SPT).
Warning
The maximum particle displacement is not automatically computed. For batch analysis of a group of files, it is recommended to fix a maximum tracking displacement on a test file and to run recipes by batch.