Cell Motility

(requires: Advanced 2D Tracking)

This analysis measures cell motility. A simple timelapse-only ND2 file must be open for this command to be enabled. The results of the analysis are automatically loaded into the Tracking panel (see Graph, Data and Tracks tabs).

Options

Recipe

Select which recipe (a collection of settings) is used for the task from the pull down menu. You can also Load another recipe you have previously created or get. When you made some changes to the recipe, you can either Save them to the currently selected recipe, or Save As a new recipe.

Channel

Select which channel is analysed.

Color

Choose color of the binary layer.

Threshold/Bright Spot Detection/Dark Spot Detection

For Threshold, use the sliders to define the thresholded objects. You can also manually overwrite the values of the thresholding limits.

For more information about Bright Spot Detection / Dark Spot Detection, please see Spot Detection.

Binary operations

You can apply some of the binary operations to improve the thresholded binary image.

Restrictions

You can filter the detected binary objects using the Size or Circularity restriction filters.

Run on

Select which dimension is used for the analysis.

Preview

Check to display preview of the result.

Run Now

Starts the task and displays the View > Analysis Controls > Automated Measurement Results window and the Measure > Time Series Graph with the results.

Cancel

Cancels the task and closes the window.