Region Settings tools and settings

Channel Settings

In the first three options, Autosignal.ai collects values (power and exposition) on automatically generated focus points and generates ideal experiment settings.

Per Slide

Procedure is repeated for each slide individually.

Per Region

Procedure is repeated for each region individually.

On First Slide

Values acquired on the first slide will be propagated to all other slides.

Current

Current experiment settings will be used on all slides.

Brightfield Settings

Select a predefined experiment by clicking on an experiment button. When hovering over a button, the experiment objective, zoom, power and exposure settings are displayed. To adjust the default brightness experiment (exposure), right mouse button click the button and choose Edit default settings. To add a new brightfield experiment, click Create a new brightfield experiment and give it a name. To delete an experiment, right mouse button on it and select Delete.

Experiment Settings

Select a fluorescence experiment by clicking on its button. To add a new one, click Add a new experiment, type its name in the search field and select it from the search list. Then adjust its name, emission and color, and modify the name of the new button. Optionally, select the Use Brightfield Channel box to capture the brightfield channel too and confirm it by clicking Finish. To include a brightfield channel click and confirm Try all wavelengths. To delete an experiment, right mouse button on it and select Delete.

Sample Type

Flat

This option is designed for thin, planar samples. When selected, the system utilizes a flat focus surface that automatically compensates for any tilt in the specimen to ensure a sharp image across the entire slide.

Uneven

This setting is intended for curved or irregular surfaces. It operates by creating a mesh of multiple focus points that approximate the specific 3D shape of the sample. Users can further customize the Focus surface density and set an upper limit for the number of focus points used.

Thick

This option is used for highly uneven or deep samples. It employs EDF (Extended Depth of Focus) to acquire a stack of images at different heights and combine them into a single focused result. The user can manually define the EDF Range to be applied around the detected focus position.

Split

Turns on a special view, where color channels of the image are displayed separately.

Shading Correction

This window provides tools to correct uneven lighting. User can select between the Automatic mode, which captures a 6 x 6 frame grid, or Advanced mode for manual control. The Create New switch allows calibration to be performed either Per Slide for individual accuracy or On First Slide to apply a single profile to a full slide batch.

The Acquire on setting determines the source of the calibration data, allowing the system to sample from either the Tissue (inside the sample) or the Background (outside the mask). Reset Shading... option is used for deleting all existing shading maps.