Append an arbitrary file to the currently opened image document. The Select Attachment window appears, where you can specify the appended file.

This command appends the selected (visible) binary layers to the original contents of the Graticule mask.

Inserts a new profile line.

Appends a line to the current multipoint experiment table including the PFS offset.

Applies the LUTs settings to the current channel.

This function changes color of any existing binary layer.

Opens the Brightfield Opacity dialog window. Define opacity of the brightfield image in the window.

This command changes the Z order of all vector objects so the selected ones are brought to front.

This command inserts the current scale layer into the color image. The scale becomes part of the image. You will be prompt to select whether to process the current image, selected dimension, or the whole ND document.

This command sets calibration with the mentioned objective to the current document in micrometers/pixel. Micrometers are expected as Units.

Erases all vector overlay layers of the current image i.e. annotations, measurement objects, ROIs.

Removes all vector objects from the current image.

Closes the current document.

This function displays the binary objects in several different colors. The algorithm is optimized so that two neighboring objects would never display in similar colors.

Having a Z-stack image and a 3D binary. This command colorizes the 3D objects to easily determine if two neighboring objects are connected or not. This command is available on a context menu over Volume View and Slices View.

The 3D binary is created by one of these commands:

Connects Z-stack slices of 2D binary objects into 3D binary objects. It equals the Binary > Connect 3D Objects in Binaries command and appears in the context menu when the image is displayed in Volume View.

This function copies all selected vector objects to the clipboard.

This command copies the current graticule into the current binary image.

This function copies current binary image (in case of ND) from selected (visible) layers into clipboard. The copied binary layers can be inserted into another image or as a new image.

The function copies binary layers into the clipboard. The layers can be pasted into different documents.

This command copies the current graticule into the current binary image.

Displays the Correlation Properties dialog window used for defining the correlation view.

Figure 2134. 

Turns the Pearson's correlation on and off. It is used to visually interpret the relation between two image channels and to see which areas in the image are correlated.

Start by thresholding your image (see: Thresholding) to determine the areas where the correlation will be calculated. Then define the channels and method in the Correlation Properties dialog (Correlation Properties...) and confirm the settings by clicking OK. Now run this function to calculate the correlation.

Hovering over a thresholded area shows the Pearson's correlation value on the bottom right side of the image window. It takes values from -1 to +1 (default Correlation Range), where -1 is a total negative linear correlation, 0 is a no linear correlation and 1 is a total positive linear correlation.

Moves the scale bar into its default position.

Deletes all binary layers.

This command removes the selection from all the vector objects in the current document.

Shows/hides information about user events if they are available.

Draws a background ROI having the selected shape.

This command is used for vector object editing.

This function creates a new image document by copying the currently selected components (channels). Components which were not selected are cropped.

Opens the graticule properties window and selects the Mask type. See Binary > Mask > Mask Properties. Mask will be activated after clicking the OK button.

If two images have matching XY coordinates, this function can be used to locate the position in one image inside the other one.

Fixes the background offset to the current value and stops updating it by values retrieved from the background ROIs.

This function removes unselected components (channels) from the current image. Only the selected ones remain.

Sets the option if the background offset should be kept updated using the values retrieved from the background ROIs on the current image.

Unlocks the selected ROIs.

This command locks/unlocks the currently selected vector objects. It prevents the user from moving them by mistake.

Locks/unlocks the scale bar in its position.

This command moves the image probe to the center of the screen.

Centers the XY stage to the selected point.

This command restores the default position of the current grid.

Moves the stage(s) to a specified Z position.

Displays a dialog used for customizing the shown ND information.

Sets visibility of the currently selected annotation/measurement object(s) over time.

See Working with the Measurement and Annotation Objects.

Opens the file attached to the currently opened document.

Opens the current image in Organizer (View > Visualization Controls > Organizer ).

Displays a dialog window which selects a file with the graticule, previously exported via the Binary > Mask > Save Mask command.

Sets color used to highlight over-saturated pixels within the image.

This function inserts all vector objects that are currently in the application clipboard to the current image. Some vector objects should have been copied to the clipboard previously.

The function pastes binary layers from clipboard into current document. Clipboard must contain some binary layer.

This function displays the vector object properties dialog window of the currently selected object(s). When more objects are selected at once, only the shared properties are displayed.

Displays a dialog window where the channels present in the custom channel can be selected, autoscaled or extracted.

Figure 2135. 

Shows a dialog where the properties (name, modality, wavelength and color) of a specified channel can be changed.

This function deletes the currently selected vector objects from the image.

Removes the specified channel (component) from the current image.

This function removes the attached file.

Opens the Reorder Channels dialog window enabling to change the order of the channels in the currently selected image.

This function resets all changes made in the volume view into default.

Resets the offset calculated by the background probe and switches the background probe off.

Removes LUTs settings of the current channel.

Sets the selected type of picture rescaling method.

Sets the camera settings to match the settings of the specified image file. The *.TIF, *.ND2 and *.JP2 file formats are supported. This function is invoked on the context menu in Opened Images and Auto Capture Folder control windows.

Loads device-related metadata from an image file and sets the connected device(s) accordingly. The *.TIF, *.ND2 and *.JP2 file formats are supported.

Sets the ND setup to match the settings of the specified ND image file. The *.TIF and *.ND2 file formats are supported. This function is invoked on context menu in 'Auto Capture Folder' and 'Opened Images' control windows and also on the 'Reuse' button in 'Image Properties' dialog box.

Optical configuration is taken from the image and is reused.

This command opens the ROI Properties dialog window where visual parameters of the ROIs can be set.

Saves the attached file to a custom location.

Saves the current graticule mask. A Save As Image dialog box is shown.

Defines properties of the scale bar.

This command is used for selecting a camera.

Selects all vector objects.

This command changes the Z order of all vector objects so the selected ones are sent to back.

Removes the current image calibration. The image becomes Uncalibrated.

This function displays/hides ID labels of the binary objects.

Shows or hides the LUT visualization bar. LUT visualization bar is overlaid on top of the image.

This function displays summarized ND information (information about the current frame of an ND2 document or live image).

Displays value of physical zoom (real magnification of the sample) in a box in the top left corner of the image. To hide the box, right-click on it and select Hide Physical Zoom.

Turns on/off visibility of the scroll bars when the image is zoomed in.

Sets the option whether the sub-view with all channels should be present in the Split Components viewing mode.

Sets the option whether the Custom sub-view should be present in the Split Components viewing mode.

All channels currently selected for viewing will be saved to the Custom tab. The previous settings will be overwritten.

Sets color used to highlight under-saturated pixels within the image.

All multipoints present in the image are shown in the preview of the XYZ Overview panel.

Currently selected multipoint is shown in the preview of the XYZ Overview panel.

A single channel of an image can be observed as gray or colored. This function toggles between these two modes. The setting is applied to the current image only.

This function sets the appearance and behaviour of image toolbars.

Sets the selected transparency of the overlay image.

Changes the projection used for viewing the ND2 file. Can be used on maximum/minimum intensity views, Slices view and Tiled view. Not all projection types are available on each view.