
Append an arbitrary file to the currently opened image document. The Select Attachment window appears, where you can specify the appended file.

Appends a line to the current multipoint experiment table including the PFS offset.

Erases all vector overlay layers of the current image i.e. annotations, measurement objects, ROIs.


Having a Z-stack image and a 3D binary. This command colorizes the 3D objects to easily determine if two neighboring objects are connected or not. This command is available on a context menu over Volume View and Slices View.
The 3D binary is created by one of these commands:

This function displays the binary objects in several different colors. The algorithm is optimized so that two neighboring objects would never display in similar colors.

(requires: Standard)
Connects Z-stack slices of 2D binary objects into 3D binary objects. It equals the Binary > Connect 3D Objects in Binaries command and appears in the context menu when the image is displayed in Volume View.

This function copies current binary image (in case of ND) from selected (visible) layers into clipboard. The copied binary layers can be inserted into another image or as a new image.

The function copies binary layers into the clipboard. The layers can be pasted into different documents.

Displays the Correlation Properties dialog window used for defining the correlation view.
Applies the current correlation settings without closing the dialog window.
Discards all the changes and closes the dialog window.
Set between which channels will the correlation be computed.
Changes the correlation range (minimum and maximum values). Click
to determine a proper range automatically.Displays this help page.
If checked, the color scale stays in view when the image is zoomed in.
Confirms the settings and closes the dialog window.
Select a correlation method:
Binary Objects - computes the Pearson's correlation coefficient for each binary object.
Neighborhood - Pearson's correlation coefficient is computed for a square neighborhood of each pixel. Define the neighborhood size in pixels using the spin box.
Pixel Deviations - is calculated as a covariance per pixel.

Turns the Pearson's correlation on and off. It is used to visually interpret the relation between two image channels and to see which areas in the image are correlated.
Start by thresholding your image (see: Thresholding) to determine the areas where the correlation will be calculated. Then define the channels and method in the Correlation Properties dialog (Correlation Properties...) and confirm the settings by clicking . Now run this function to calculate the correlation.
Hovering over a thresholded area shows the Pearson's correlation value on the bottom right side of the image window. It takes values from -1 to +1 (default Correlation Range), where -1 is a total negative linear correlation, 0 is a no linear correlation and 1 is a total positive linear correlation.

If two images have matching XY coordinates, this function can be used to locate the position in one image inside the other one.

Sets visibility of the currently selected annotation/measurement object(s) over time.

Clears the frame selection.

Applies the visibility and closes the settings window.

Opens this help page.

Inverts the frame selection.

Selects all frames of the image.

Opens the current image in Organizer ( View > Visualization Controls > Organizer
).

The function pastes binary layers from clipboard into current document. Clipboard must contain some binary layer.

This function inserts all vector objects that are currently in the application clipboard to the current image. Some vector objects must have been copied to the clipboard previously.

Sets the camera settings to match the settings of the specified image file. The *.TIF, *.ND2 and *.JP2 file formats are supported. This function is invoked on the context menu in Opened Images and Auto Capture Folder control windows.

Loads device-related metadata from an image file and sets the connected device(s) accordingly. The *.TIF, *.ND2 and *.JP2 file formats are supported.

Sets the ND setup to match the settings of the specified ND image file. The *.TIF and *.ND2 file formats are supported. This function is invoked on context menu in 'Auto Capture Folder' and 'Opened Images' control windows and also on the 'Reuse' button in 'Image Properties' dialog box.

This command opens the ROI Properties dialog window where visual parameters of the ROIs can be set.

Removes the current image calibration. The image becomes Uncalibrated.

Shows or hides the LUT visualization bar. LUT visualization bar is overlaid on top of the image.

This function displays summarized ND information (information about the current frame of an ND2 document or live image).

Displays value of physical zoom (real magnification of the sample) in a box in the top left corner of the image. To hide the box, right-click on it and select Hide Physical Zoom.
Note
Correct monitor calibration is required by this function to work correctly. See Monitor Calibration.

Sets the option whether the sub-view with all channels should be present in the Split Components viewing mode.

Sets the option whether the Custom sub-view should be present in the Split Components viewing mode.

This function quickly changes the appearance and behavior of image toolbars. The same options are accessible in program options.
See Appearance Options.

All multipoints present in the image are shown in the preview of the XYZ Overview panel.