Threshold

AutoSegmentation

Description

 AutoSegmentation(
    LParam
);

This function automatically detects object starting from the point defined in LParam (in pixels).

Parameters

LParam

x = LOWORD(LParam), y = HIWORD(LParam)

BinarySegmentationCall

Description

 BinarySegmentationCall();

This function runs the binary segmentation. The Call Binary Segmentation dialog window appears. Select which settings is applied and run the segmentation.

BinarySegmentationDefine

Description

 BinarySegmentationDefine();

This function opens the Define Binary Segmentation dialog window. Use tools in this window to define image segmentation.

ColocalizeND

Description

 ColocalizeND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

Makes colocalization of channels on the current ND document.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ColorizeBinaryObjects

This function runs the Colorize Binary Objects command.

Description

 ColorizeBinaryObjects(
   int  VN_ColorizeBinObjects
);

This function displays the binary objects in several different colors. The algorithm is optimized so that two neighboring objects would never display in similar colors.

Parameters

int VN_ColorizeBinObjects

Colorize the binary objects?

0	Display the binary layer in one color
1	Display the binary layer in colors

ComponentThreshold

Description

 ComponentThreshold();

This function segments color image according to threshold levels defined interactively in the Define Threshold dialog window.

Note

This function detects pixels with RGB intensities within the range specified by the Define Component Threshold command. Current binary image is a result of the transformation.

ComponentThresholdND

Description

 ComponentThresholdND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function segments color image according to threshold levels defined interactively via the _DefineComponentThreshold function.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

Note

This function detects pixels with RGB intensities within the range specified by the Define Component Threshold command. Current binary image is a result of the transformation.

DefineComponentThreshold

Description

 DefineComponentThreshold(
   int  RLow,
   int  GLow,
   int  BLow,
   int  RHigh,
   int  GHigh,
   int  BHigh,
   int  RTol,
   int  GTol,
   int  BTol,
   int  RRes,
   int  GRes,
   int  BRes
);

This function defines threshold levels for multichannel thresholding method on RGB images, where binaries are created per channel and sets this method for the active document.

Parameters

int RLow

Lower intensity limit of the red image channel.

int GLow

Minimum value of the green image channel.

int BLow

Lower bound of blue threshold values.

int RHigh

Higher bound of red threshold values.

int GHigh

Maximum value of the green image channel.

int BHigh

Maximum intensity of the blue image component.

int RTol

Specifies threshold range extensions for red channel.

int GTol

Specifies threshold range extensions for green channel.

int BTol

Specifies threshold range extensions for blue channel.

int RRes

Resets red threshold values if set to TRUE (1).

int GRes

Resets green threshold values if set to TRUE (1).

int BRes

Resets blue threshold values if set to TRUE (1).

Note

This function is used for backward compatibility. This function does not perform thresholding.

DefineComponentThresholdMCh

Description

 DefineComponentThresholdMCh(
   int *MCHLowArray,
   int *MCHHighArray,
   int *MCHTolArray,
   int *MCHResetedlArray,
   int *MCHThresholdOperation
);

This function defines all threshold levels for multichannel thresholding and sets this method for the active document.

Parameters

int *MCHLowArray

The array containing threshold lower bounds' values.

int *MCHHighArray

The array containing threshold higher bounds' values.

int *MCHTolArray

The array containing threshold tolerance values.

int *MCHResetedlArray

The array containing threshold reset values. Use TRUE (1) to reset the corresponding channel.

int *MCHThresholdOperation

Sets the current operation for multichannel method.

0, THRESHOLD_OPERATION_INTERSECTION	Intersection of all the channels
1, THRESHOLD_OPERATION_BINPERCHANNEL	Binaries per channel

Note

This function is used for backward compatibility. This function does not perform thresholding.

DefineThreshold

Description

 DefineThreshold(
   int  RLow,
   int  GLow,
   int  BLow,
   int  RHigh,
   int  GHigh,
   int  BHigh,
   int  Tol
);

This function defines threshold levels for the RGB thresholding method and sets this method for the active document.

Parameters

int RLow

Lower intensity limit of the red image channel.

int GLow

Minimum value of the green image channel.

int BLow

Lower bound of blue threshold values.

int RHigh

Higher bound of red threshold values.

int GHigh

Maximum value of the green image channel.

int BHigh

Maximum intensity of the blue image component.

int Tol

Specifies threshold range extensions. If you subtract Tol from Low you get the new lower values of threshold. If you add Tol to High values you get the new higher values of threshold.

Note

This function is same as the DefineThresholdRGB function and is only used for backward compatibility. The RGB method can be used for RGB images only. This function does not perform thresholding.

DefineThresholdCalcComp

Description

 DefineThresholdCalcComp(
   char *LpChannelName,
   int  Low,
   int  High
);

This function defines calcium channel threshold.

Parameters

char *LpChannelName

Valid string for storing the channel name.

int Low

The lower intensity limit.

int High

The higher intensity limit.

See Also

_DefineThresholdCalcComp

DefineThresholdHsiGa3

Description

 DefineThresholdHsiGa3(
   double  LoH,
   double  LoS,
   double  LoI,
   double  HiH,
   double  HiS,
   double  HiI
);

This General Analysis 3 function runs the HSI Threshold.

Parameters

double LoH

Low hue value of the HSI Threshold.

double LoS

Low saturation value of the HSI Threshold.

double LoI

Low intensity value.

double HiH

High hue value of the HSI Threshold.

double HiS

High saturation value of the HSI Threshold.

double HiI

High intensity value.

DefineThresholdIHS

Description

 DefineThresholdIHS(
   int  LowH,
   int  LowS,
   int  LowI,
   int  HighH,
   int  HighS,
   int  HigI,
   int  TolH,
   int  TolS,
   int  TolI
);

This function defines threshold levels for HSI thresholding method and sets this method for the active document.

Parameters

int LowH

Lower bound of hue threshold values.

int LowS

Lower bound of saturation threshold values.

int LowI

Lower bound of intensity threshold values.

int HighH

Higher bound of hue threshold values.

int HighS

Higher bound of saturation threshold values.

int HigI

Higher bound of intensity threshold values.

int TolH

Specifies hue threshold range extensions. If you subtract TolH from LowH, you get the new lower bounds of hue threshold. If you add TolH to HighH value you get the new higher bounds of hue threshold.

int TolS

Specifies saturation threshold range extensions. If you subtract TolS from LowS, you get the new lower bounds of saturation threshold. If you add TolS to HighS value you get the new higher bounds of saturation threshold.

int TolI

Specifies intensity threshold range extensions. If you subtract TolI from LowI, you get the new lower bounds of intensity threshold. If you add TolI to HighI value you get the new higher bounds of intensity threshold.

Note

The HSI method can be used for RGB images only. To specify an empty range set lower bound to greater value than higher bound. This function does not perform thresholding.

DefineThresholdIntensity

Description

 DefineThresholdIntensity(
   int  LowI,
   int  HighI,
   int  TolI
);

This function defines threshold levels for intensity thresholding method and sets this method for the active document.

Parameters

int LowI

Lower bound of intensity threshold values.

int HighI

Higher bound of threshold intensity values.

int TolI

Note

The intensity method can be used for RGB, multichannel and mono images. To specify an empty range set lower bound to greater value than higher bound. This function does not perform thresholding.

DefineThresholdIntGa3

Description

 DefineThresholdIntGa3(
   double  LoI,
   double  HiI
);

This General Analysis 3 function runs Threshold with the Intensity method selected.

Parameters

double LoI

Low intensity value.

double HiI

High intensity value.

DefineThresholdMCHChannel

Description

 DefineThresholdMCHChannel(
   int  Channel,
   int  Low,
   int  High,
   int  Tol
);

This function defines threshold levels for multichannel thresholding method and sets this method for the active document. Channel numbers correspond to the channel tabs at the bottom of the image view starting with 0 from the left.

Parameters

int Channel

Index number of the channel.

int Low

The lower intensity limit.

int High

Higher bound of threshold values.

int Tol

Specifies threshold range extensions. If you subtract Tol from Low you get the new lower values of threshold. If you add Tol to High values you get the new higher values of threshold.

Note

The multichannel method can be used for RGB and multichannel images. To specify an empty range set lower bound to greater value than higher bound. This function does not perform thresholding.

DefineThresholdMchGa3

Description

 DefineThresholdMchGa3(
   double *LoArray,
   double *HiArray,
   int *MChResetedArray,
   int  Count
);

Opens the Define Threshold dialog window with the Multi-Channel method selected as default. Click on the help button in the dialog for more information.

Parameters

double *LoArray

Array for the low multi-channel threshold values.

double *HiArray

Array for the high multi-channel threshold values.

int *MChResetedArray

Multi-channel threshold resetted array.

int Count

Number of iterations.

DefineThresholdMchOnChannelGa3

Description

 DefineThresholdMchOnChannelGa3(
   int  Channel,
   double  ChLo,
   double  ChHi
);

This General Analysis 3 function defines threshold levels for multichannel thresholding method.

Parameters

int Channel

Index number of the channel.

double ChLo

Low threshold value.

double ChHi

High threshold value.

DefineThresholdMCHOperation

Description

 DefineThresholdMCHOperation(
   int  ThresholdOperation
);

This function selects multichannel method for the active document and defines its thresholding operation.

Parameters

int ThresholdOperation

Sets the current operation for multichannel method.

0, THRESHOLD_OPERATION_INTERSECTION	Intersection of all the channels
1, THRESHOLD_OPERATION_BINPERCHANNEL	Binaries per channel

Note

Multichannel method can be used for RGB and multichannel images. This function does not perform thresholding.

DefineThresholdMethod

Description

 DefineThresholdMethod(
   int  ThresholdMethod
);

This function sets a thresholding method for the active document.

Parameters

int ThresholdMethod

The threshold method number.

0, THRESHOLD_METHOD_RGB	RGB method
2, THRESHOLD_METHOD_MCH	Multichannel method
1, THRESHOLD_METHOD_HSI	HSI method
3, THRESHOLD_METHOD_INTENSITY	Intensity method

Note

RGB and HSI method can be used for RGB images only. Multichannel method can be used for RGB and multichannel images. The RGB method always performs intersection of all the channels. The multichannel method can either perform intersection of all the channels or create binaries per channel according to the currently selected multichannel operation. This function does not perform thresholding.

DefineThresholdProcessing

Description

 DefineThresholdProcessing(
   int  ThresholdClean,
   int  ThresholdSmooth,
   int  ThresholdSeparate,
   int  ThresholdFillHoles
);

This function defines threshold processing parameters for the active document. Its parameters define the number of morphology operations to be performed after thresholding.

Parameters

int ThresholdClean

Clean parameter.

0	OFF
N, 1-inf	Number of iterations.

int ThresholdSmooth

Smooth parameter.

0	OFF
N	Number of iterations

int ThresholdSeparate

Separate parameter.

0	OFF
N	Number of iterations

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

Note

The actual values that will be set to the document may differ due to some limitations. This function does not perform thresholding.

DefineThresholdProcessingGa3

Description

 DefineThresholdProcessingGa3(
   double  SmoothSize,
   double  CleanSize,
   int  ThresholdFillHoles,
   double  SeparateSize
);

This General Analysis 3 function defines threshold processing parameters for the active document. Its parameters define the number of morphology operations to be performed after thresholding.

Parameters

double SmoothSize

Smooth processing size [px].

double CleanSize

Clean processing size [px].

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

double SeparateSize

Separate processing size [px].

DefineThresholdProcessingOnChannel

Description

 DefineThresholdProcessingOnChannel(
   int  Channel,
   int  ThresholdClean,
   int  ThresholdSmooth,
   int  ThresholdSeparate,
   int  ThresholdFillHoles
);

Defines threshold processing parameters on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

int ThresholdClean

Clean parameter.

0	OFF
N, 1-inf	Number of iterations.

int ThresholdSmooth

Smooth parameter.

0	OFF
N	Number of iterations

int ThresholdSeparate

Separate parameter.

0	OFF
N	Number of iterations

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

Note

The actual values that will be set to the document may differ due to some limitations. This function does not perform thresholding.

DefineThresholdProcessingOnChannelGa3

Description

 DefineThresholdProcessingOnChannelGa3(
   int  Channel,
   double  SmoothSize,
   double  CleanSize,
   int  ThresholdFillHoles,
   double  SeparateSize
);

This General Analysis 3 function defines the threshold processing parameters on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

double SmoothSize

Smooth processing size [px].

double CleanSize

Clean processing size [px].

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

double SeparateSize

Separate processing size [px].

DefineThresholdRGB

Description

 DefineThresholdRGB(
   int  RLow,
   int  GLow,
   int  BLow,
   int  RHigh,
   int  GHigh,
   int  BHigh,
   int  Tol
);

This function defines threshold levels for the RGB thresholding method and sets this method for the active document.

Parameters

int RLow

Lower intensity limit of the red image channel.

int GLow

Minimum value of the green image channel.

int BLow

Lower bound of blue threshold values.

int RHigh

Higher bound of red threshold values.

int GHigh

Maximum value of the green image channel.

int BHigh

Maximum intensity of the blue image component.

int Tol

Specifies threshold range extensions. If you subtract Tol from Low you get the new lower values of threshold. If you add Tol to High values you get the new higher values of threshold.

Note

The RGB method can be used for RGB images only. To specify an empty range set the lower bound to a value greater than the higher bound. This function does not perform thresholding.

DefineThresholdRgbGa3

Description

 DefineThresholdRgbGa3(
   double  LoR,
   double  LoG,
   double  LoB,
   double  HiR,
   double  Hig,
   double  HiB,
   double  TolD
);

This General Analysis 3 function runs the RGB Threshold.

Parameters

double LoR

Low red value of the RGB Threshold.

double LoG

Low green value of the RGB Threshold.

double LoB

Low blue value of the RGB Threshold.

double HiR

High red value of the RGB Threshold.

double Hig

High green value of the RGB Threshold.

double HiB

High blue value of the RGB Threshold.

double TolD

Tolerance value of the RGB Threshold.

DefineThresholdRestrictionCircularity

Description

 DefineThresholdRestrictionCircularity(
   bool  RestrictionEnable,
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh
);

This function defines circularity restriction for the active document. The restriction is used for thresholding.

Parameters

bool RestrictionEnable

Enables/disables restriction.

0, FALSE	Disable restriction
1, TRUE	Enable restriction

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

Note

This function does not perform thresholding.

DefineThresholdRestrictionCircularityGa3

Description

 DefineThresholdRestrictionCircularityGa3(
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh
);

This General Analysis 3 function defines circularity restriction for the active document. The restriction is used for thresholding.

Parameters

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

DefineThresholdRestrictionCircularityOnChannel

Description

 DefineThresholdRestrictionCircularityOnChannel(
   int  Channel,
   int  RestrictionCircularityEnable,
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh
);

This function defines the circularity restriction on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

int RestrictionCircularityEnable

Exclude objects which do not match the Circularity limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

Note

This function does not perform thresholding.

DefineThresholdRestrictionCircularityOnChannelGa3

Description

 DefineThresholdRestrictionCircularityOnChannelGa3(
   int  Channel,
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh
);

This General Analysis 3 function defines the circularity restriction on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

DefineThresholdRestrictionSize

Description

 DefineThresholdRestrictionSize(
   bool  RestrictionEnable,
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh
);

This function defines the size restriction for the active document. The restriction is used for thresholding.

Parameters

bool RestrictionEnable

Enables/disables restriction.

0, FALSE	Disable restriction
1, TRUE	Enable restriction

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

Note

This function does not perform thresholding.

DefineThresholdRestrictionSizeGa3

Description

 DefineThresholdRestrictionSizeGa3(
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh
);

This General Analysis 3 function defines the size restriction for the active document. The restriction is used for thresholding.

Parameters

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

DefineThresholdRestrictionSizeOnChannel

Description

 DefineThresholdRestrictionSizeOnChannel(
   int  Channel,
   int  RestrictionSizeEnable,
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh
);

This function defines the size restriction on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

int RestrictionSizeEnable

Exclude objects which do not match the Size limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

Note

This function does not perform thresholding.

DefineThresholdRestrictionSizeOnChannelGa3

Description

 DefineThresholdRestrictionSizeOnChannelGa3(
   int  Channel,
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh
);

This General Analysis 3 function defines the size restriction on the specified channel of the active document.

Parameters

int Channel

Index number of the channel.

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

EnableDynamicThreshold

Description

 EnableDynamicThreshold(
   int  EnableDynamicThreshold
);

This function enables/disables the dynamic threshold.

Parameters

int EnableDynamicThreshold

Dynamic threshold.

0	Disable dynamic threshold
1	Enable dynamic threshold

GBasedSegmentation

Interactive command to this function: Binary > Segmentation > Graph Cut Segmentation

Description

 GBasedSegmentation(
   int  NDDimension
);

Executes the Graph Cut Segmentation on the selected dimension.

Parameters

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

See Also

_GBasedSegmentation

GetOtsuThreshold

Description

 GetOtsuThreshold();

This macro function selects the optimal threshold extent for the given image.

HomogeneousSegmentation

Interactive command to this function: Binary > Segmentation > Homogeneous Area Detection

Description

 HomogeneousSegmentation(
   int  HomogeneousSegmentationLOD,
   int  HomogeneousSegmentationInversion,
   int  HomogeneousSegmentationTT1,
   int  HomogeneousSegmentationTT2,
   int  HomogeneousSegmentationType,
   int  NDDimension
);

Applies the Homogeneous Area Segmentation.

Parameters

int HomogeneousSegmentationLOD

Level of Details value.

int HomogeneousSegmentationInversion

Inverts the detected binary.

0	Inversion OFF.
1	Inversion ON.

int HomogeneousSegmentationTT1

Compactness of the Homogeneous Area Detection.

int HomogeneousSegmentationTT2

Threshold of the Homogeneous Area Detection.

int HomogeneousSegmentationType

Type of the Homogeneous Area Detection (Edge Based or Variance Based method).

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

LineDetection

Description

 LineDetection(
   double  RelativeThresholdLimit,
   double  LineThicknessDbl,
   double  AngleResolution,
   double  MinimalLineLength,
   int  ProcessComponent,
   int  NDDimension
);

This function thresholds the current image and detects lines using Hough transform. Detected lines are saved into the binary layer each line becoming a single object.

Parameters

double RelativeThresholdLimit

Range <0,1> sets the upper threshold bound.

double LineThicknessDbl

Expected line thickness [px].

double AngleResolution

Precision of determining the slope of a line [°].

double MinimalLineLength

Minimal length of a found line [px].

int ProcessComponent

Selection of color components.

1	The first component (Blue in RGB image, binary 1)
2	The second component (Green in RGB image, binary 10)
4	The third component (Red in RGB image, binary 100)
7	The first three components (All RGB, binary 111)

Note

The “Brightfield” channel consists of 3 components.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

LoadThresholdGa3

Description

 LoadThresholdGa3(
   char *ThresholdFileName
);

Loads the settings of the General Analysis 3 Threshold dialog from the specified file.

Parameters

char *ThresholdFileName

Defines complete path to the file you want to save/load.

ObjectCountThresholdND

Description

 ObjectCountThresholdND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function thresholds the current image. If performed on an ND document, the parameters will be taken into account, otherwise they will be ignored.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ResetThresholdGa3

Description

 ResetThresholdGa3();

Resets the General Analysis 3 threshold.

ResetThresholdIHS

Description

 ResetThresholdIHS(
   int  ResetedIHS
);

This function sets the flag of HSI threshold procedure to the resetted state.

Parameters

int ResetedIHS

0	Sets the thresholding mode to [HSI].
1	Thresholded image with one typical point of the following color coordinates: Hue = 0, Saturation =0, Intensity = 0, Hue Tolerance = 0, Saturation Tolerance = 0 and Intensity Tolerance = 0.

Note

Note, that the flag distinguish two different states: 1) start of thresholding after reset 2) thresholded image with one typical point of the following color coordinates: Hue = 0, Saturation =0, Intensity = 0, Hue Tolerance = 0, Saturation Tolerance = 0 and Intensity Tolerance = 0.

See Also
ThresholdIHS

SaveThresholdGa3

Description

 SaveThresholdGa3(
   char *ThresholdFileName
);

Saves the settings of the General Analysis 3 Threshold dialog into the specified file.

Parameters

char *ThresholdFileName

Defines complete path to the file you want to save/load.

SegmentTightBorders

This function runs the Binary > Segmentation > Segment Tight Borders command.

Description

 SegmentTightBorders();

Performs the segmentation of binary layer using tight borders detection.

ShowBinaryObjectID

This function runs the Show Binary Object ID command.

Description

 ShowBinaryObjectID(
   int  ShowBinIDs
);

This function displays/hides ID labels of binary objects.

Parameters

int ShowBinIDs

State of the control.

0	Hides binary ID
1	Displays binary ID

SpotDetection3DLoadSettings

Description

 SpotDetection3DLoadSettings(
   char *SpotDetectionFileName
);

Loads the settings from the .spotdetection3D file into the 3D Spot Detection dialog window.

Parameters

char *SpotDetectionFileName

Path where the .spotdetection3D file is stored.

SpotDetection3DSaveSettings

Description

 SpotDetection3DSaveSettings(
   char *SpotDetectionFileName
);

Opens the Save Spot Detection Settings dialog window used for exporting the 3D Spot Detection settings to a .spotdetection3D file.

Parameters

char *SpotDetectionFileName

Path where the .spotdetection3D file is stored.

SpotDetectionLoadSettings

Description

 SpotDetectionLoadSettings(
   char *SpotDetectionFileName
);

Loads the settings from the .spotdetection file into the Spot Detection dialog window.

Parameters

char *SpotDetectionFileName

Path where the .spotdetection file is stored.

SpotDetectionSaveSettings

Description

 SpotDetectionSaveSettings(
   char *SpotDetectionFileName
);

Saves the Spot Detection dialog window settings into a .spotdetection file.

Parameters

char *SpotDetectionFileName

Path where the .spotdetection file is stored.

SpotDetection

Description

 SpotDetection(
   double  EqDiameter,
   double  Contrast,
   double  Quantile,
   int  RestrictIntensity,
   int  GrowingThreshold,
   int  ProcessComponent,
   int  SpotSize,
   int  Kernel,
   int  UseIntensity,
   int  Inverted,
   int  CreatePoints,
   int  CreateCircles,
   int  GrowPoints,
   int  NDDimension
);

Runs spot detection on one color component of the current image.

Please see Spot Detection.

Parameters

double EqDiameter

Adjusts the size of your object.

double Contrast

Contrast value calculated as a difference between mean intensity inside and mean intensity outside the spot. All the spots with Contrast higher than this value are included into the result.

double Quantile

Quantile (contrast) of each spot is computed as a difference between mean spot intensity and mean of the voxels outside the spot (in the close vicinity) that have intensity greater than 100-quantile % of the pixels in the neighborhood (100-quantile brightest pixels). All the spots with quantile (contrast) higher than contrast are included into the result. Quantile value ranges from 0 to 100.

Note

If quantile is 0, contrast behaves as defined before. This is valid for bright spots, for dark spots the values are computed inversely.

int RestrictIntensity

Intensity value calculated as mean intensity of the spot. All the spots with (mean) intensity higher than this value are included into the result.

int GrowingThreshold

Growing threshold is used in growing. It is the lowest (for bright spots) intensity value included into the grown region.

int ProcessComponent

Selection of color components.

1	The first component (Blue in RGB image, binary 1)
2	The second component (Green in RGB image, binary 10)
4	The third component (Red in RGB image, binary 100)
7	The first three components (All RGB, binary 111)

Note

The “Brightfield” channel consists of 3 components.

int SpotSize

Spot spinner value.

int Kernel

Shape of the neighborhood defines pixels which will be taken into account.

6
26

int UseIntensity

Use intensity is a switch that can turn on and off the Intensity value restriction.

TRUE	Intensity restriction is applied.
FALSE	Intensity restriction is not used.

int Inverted

Color detection method.

0	Bright Spot.
1	Dark Spot.

int CreatePoints

Creates Spots binary.

0	Spots OFF.
1	Spots ON.

int CreateCircles

Creates Circles binary.

0	Circles OFF.
1	Circles ON.

int GrowPoints

Creates Grown Regions binary.

0	Grown Regions OFF.
1	Grown Regions ON.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

Threshold

Description

 Threshold();

This function performs threshold segmentation on the active document according to previously defined threshold method, levels, preprocessing and restriction settings.

Threshold3D

Description

 Threshold3D(
   int  Channel,
   double  LowDouble,
   int  HighDouble,
   int  Smooth,
   int  Clean,
   int  FillHoles,
   char *BinLayerNameDst,
   int  NDDimension
);

This function 3D thresholds the currently opened image document.

Parameters

int Channel

Index number of the channel.

double LowDouble

The lower intensity limit.

int HighDouble

The high threshold limit.

int Smooth

Use Smooth N-times.

OFF

int Clean

Use Clean N-times.

OFF

int FillHoles

Use Fill Holes N-times.

OFF

char *BinLayerNameDst

Name of the destination binary layer(s) which will be created by the function.

Note

Some functions accept a list of binary layers. A coma-separated list of names is expected. If the list is in another format than expected or NULL, default names will be used.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

See Also
_DefineThreshold3D, Threshold3D

ThresholdAndCopyOriginal

Description

 ThresholdAndCopyOriginal(
   int  UndoIndex
);

This function performs threshold on the current image frame and then copies a color image from a specified undo level to the current.

Parameters

int UndoIndex

Undo ID of the specified undo level.

ThresholdAndCopyOriginalND

Description

 ThresholdAndCopyOriginalND(
   int  UndoIndex,
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function performs threshold on the selected frames and then copies a color image frames from a selected undo level to the current.

Parameters

int UndoIndex

Undo ID of the specified undo level.

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

See Also
Threshold, ThresholdAndCopyOriginal

ThresholdAuto

Description

 ThresholdAuto(
   int  ThresholdOtsuSwitch,
   int  ThresholdOtsuLowHi,
   int  ThresholdOtsuNewAlg,
   int  Channel,
   int  NDDimension
);

Automatically performs threshold on the current image using the specified parameters.

Parameters

int ThresholdOtsuSwitch

Otsu threshold works well on images with balanced intensities (bright and dark areas are of similar size). If the intensities are not balanced, it must be compensated.

0	The image is well-balanced.
1	Turn on the software compensation.

int ThresholdOtsuLowHi

0	Detect dark objects
1	Detect bright objects

int ThresholdOtsuNewAlg

New thresholding algorithm.

int Channel

Index number of the channel.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

ThresholdCalcComp

Description

 ThresholdCalcComp();

This function thresholds the calcium component.

ThresholdCalcCompND

Description

 ThresholdCalcCompND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function thresholds the calcium component in an ND document.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ThresholdEdgesII

Description

 ThresholdEdgesII();

This function performs edges thresholding according to the definition.

ThresholdGa3

Description

 ThresholdGa3(
   int  NDDimension
);

This General Analysis 3 function performs threshold on the specified dimension.

Parameters

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

ThresholdIHS

Description

 ThresholdIHS();

This function sets HSI as the current thresholding method and performs threshold segmentation on the active document according to previously defined threshold method, levels, preprocessing and restriction settings.

ThresholdIHSND

Description

 ThresholdIHSND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function sets HSI as the current thresholding method and performs threshold segmentation on an ND document according to previously defined threshold method, levels, preprocessing and restriction settings.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ThresholdLoadSettings

Description

 ThresholdLoadSettings(
   char *ThresholdFileName
);

This function loads the current threshold settings from a specified file.

Parameters

char *ThresholdFileName

Defines complete path to the file you want to save/load.

ThresholdND

Description

 ThresholdND(
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function performs threshold segmentation on the active document according to the previously defined threshold method, levels, preprocessing and restriction settings.

Parameters

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ThresholdOnComponent

Description

 ThresholdOnComponent(
   int  Channel,
   char *BinLayerNameDst,
   int  Low,
   int  High,
   int  ThresholdClean,
   int  ThresholdSmooth,
   int  ThresholdFillHoles,
   int  ThresholdSeparate,
   int  RestrictionSizeEnable,
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh,
   int  RestrictionCircularityEnable,
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh
);

This function performs threshold on a single image channel (component). A new binary layer will be created.

Parameters

int Channel

Index number of the channel.

char *BinLayerNameDst

Name of the destination binary layer(s) which will be created by the function.

Note

Some functions accept a list of binary layers. A coma-separated list of names is expected. If the list is in another format than expected or NULL, default names will be used.

int Low

The lower intensity limit.

int High

High threshold limit.

int ThresholdClean

Clean parameter.

0	OFF
N, 1-inf	Number of iterations.

int ThresholdSmooth

Binary layer post-processing.

0	OFF
1-inf	Number of repetitions

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

int ThresholdSeparate

Binary layer post-processing.

0	OFF
1-inf	Number of repetitions

int RestrictionSizeEnable

Exclude objects which do not match the Size limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

int RestrictionCircularityEnable

Exclude objects which do not match the Circularity limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

See Also
ThresholdOnComponentND

ThresholdOnComponentND

Description

 ThresholdOnComponentND(
   int  Channel,
   char *BinLayerNameDst,
   int  Low,
   int  High,
   int  ThresholdClean,
   int  ThresholdSmooth,
   int  ThresholdFillHoles,
   int  ThresholdSeparate,
   int  RestrictionSizeEnable,
   double  RestrictionSizeLow,
   double  RestrictionSizeHigh,
   int  RestrictionCircularityEnable,
   double  RestrictionCircularityLow,
   double  RestrictionCircularityHigh,
   int  NDTLoop,
   int  NDMPLoop,
   int  NDZLoop
);

This function performs threshold on a single image channel (component) over the specified ND dimension. A new binary layer will be created.

Parameters

int Channel

Index number of the channel.

char *BinLayerNameDst

Name of the destination binary layer(s) which will be created by the function.

Note

Some functions accept a list of binary layers. A coma-separated list of names is expected. If the list is in another format than expected or NULL, default names will be used.

int Low

The lower intensity limit.

int High

High threshold limit.

int ThresholdClean

Clean parameter.

0	OFF
N, 1-inf	Number of iterations.

int ThresholdSmooth

Binary layer post-processing.

0	OFF
1-inf	Number of repetitions

int ThresholdFillHoles

Fill Holes binary layer post-processing.

0	OFF
1	ON
1-inf	Number of repetitions

int ThresholdSeparate

Binary layer post-processing.

0	OFF
1-inf	Number of repetitions

int RestrictionSizeEnable

Exclude objects which do not match the Size limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionSizeLow

size minimum

The low size limit (minimum) for an object to be thresholded (pixels/microns).

double RestrictionSizeHigh

The high size limit - maximum value of size. (pixels/microns)

int RestrictionCircularityEnable

Exclude objects which do not match the Circularity limits from the result?

0	No restrictions
1	Apply restrictions

double RestrictionCircularityLow

Minimum value of circularity.

double RestrictionCircularityHigh

Maximum value of circularity.

int NDTLoop

0	apply to the current loop only
1	apply to the whole Time dimension

int NDMPLoop

Apply to Multi Point dimension.

0	apply to the current loop only
1	apply to the whole Multi Point dimension

int NDZLoop

Apply to Z dimension

0	apply to the current loop only
1	apply to the whole Z Series dimension

ThresholdOtsu

Interactive command to this function: Binary > Auto Threshold

Description

 ThresholdOtsu(
   int  ThresholdOtsuSwitch,
   int  ThresholdOtsuLowHi,
   int  Channel,
   int  NDDimension
);

Performs threshold on the current image using the “Otsu threshold” algorithm. The threshold is automatic.

See Binary > Auto Threshold.

Parameters

int ThresholdOtsuSwitch

Otsu threshold works well on images with balanced intensities (bright and dark areas are of similar size). If the intensities are not balanced, it must be compensated.

0	The image is well-balanced.
1	Turn on the software compensation.

int ThresholdOtsuLowHi

0	Detect dark objects
1	Detect bright objects

int Channel

Index number of the channel.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

ThresholdSaveSettings

Description

 ThresholdSaveSettings(
   char *ThresholdFileName
);

This function saves the current threshold settings into a file.

Parameters

char *ThresholdFileName

Defines complete path to the file you want to save/load.

WoundSegmentation

Interactive command to this function: Binary > Segmentation > Wound Area Detection

Description

 WoundSegmentation(
   int  WSCloseHoles,
   int  WSCloseHolesCount,
   int  WSDoPostProcessing,
   int  WSCleanObjects,
   int  WSCleanObjectsCount,
   int  NDDimension
);

This function performs the wound area detection.

Parameters

int WSCloseHoles

Turns on/off the close holes command.

0	Close Holes OFF
1	Close Holes ON

int WSCloseHolesCount

Specifies how many times the fill holes in detected objects command will be run.

int WSDoPostProcessing

Performs logical AND with the previous result (binary layer). Only pixels which were detected in both runs will remain in the binary layer.

0	PostProcessing Area Intersection OFF
1	PostProcessing Area Intersection ON

int WSCleanObjects

Turns the Delete Small Objects command on/off.

0	Delete Small Objects OFF
1	Delete Small Objects ON

int WSCleanObjectsCount

Specifies how many times the Delete Small Objects command will be run.

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

WoundSegmentation3D

Description

 WoundSegmentation3D(
   int  WSCloseHoles,
   int  WSCloseHolesCount,
   int  WSCleanObjects,
   int  WSCleanObjectsCount,
   int  WSThreshold,
   int  WSThresholdAuto,
   int  NDDimension
);

This command performs the wound area detection in time.

Parameters

int WSCloseHoles

Turns on/off the close holes command.

0	Close Holes OFF
1	Close Holes ON

int WSCloseHolesCount

Specifies how many times the fill holes in detected objects command will be run.

int WSCleanObjects

Turns the Delete Small Objects command on/off.

0	Delete Small Objects OFF
1	Delete Small Objects ON

int WSCleanObjectsCount

Specifies how many times the Delete Small Objects command will be run.

int WSThreshold

Defines the manual wound area detection threshold.

int WSThresholdAuto

Turns on/off the automatic wound area detection threshold.

0	Auto Threshold OFF
1	Auto Threshold ON

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

_ComponentThreshold

Description

 _ComponentThreshold();

Opens the component threshold window.

_DeepTrain

Description

 _DeepTrain();

This function is not used.

_DefineComponentThreshold

Description

 _DefineComponentThreshold();

This function shows a dialog box to define and perform threshold segmentation with multichannel method and binaries per channel operation selected as default.

Note

This function is used for backward compatibility.

_DefineGraticuleMask

This function runs the Binary > Mask > Define Mask by Threshold command.

Description

 _DefineGraticuleMask();

Opens the Define Graticule Mask dialog window.

See Also
DefineGraticuleMask

_DefineThreshold

Description

 _DefineThreshold();

This function shows a dialog box to define and perform threshold segmentation.

Note

Threshold values can be defined either by using Low and High scroll bars or interactively by marking pixels with “typical color (gray)” with the mouse (left button). Interactive marking of typical points is closed by pushing secondary mouse button. When defining threshold levels you can swap color (gray) and overlay mode by pressing secondary mouse button inside the picture. Threshold levels are stored in the Windows registry file in the key HKEY_CURRENT_USER\Software\Laboratory Imaging\LUCIAG5. When calling this function, the application gets the overlay mode.

_DefineThreshold3D

This function runs the Binary > Define 3D Threshold command.

Description

 _DefineThreshold3D();

This function opens the 3D Threshold dialog window.

See Also
Threshold3D

_DefineThresholdX

This function runs the Binary > Define Threshold command.

Description

 _DefineThresholdX();

Opens the Define Threshold dialog window.

_DefineThresholdAll

Description

 _DefineThresholdAll();

This function opens the Define Threshold window

_DefineThresholdCalcComp

Description

 _DefineThresholdCalcComp();

This function opens the dialog window which defines the calcium channel threshold.

See Also

DefineThresholdCalcComp

_DefineThresholdEdgesII

This function runs the Binary > Segmentation > Threshold Edges command.

Description

 _DefineThresholdEdgesII();

This function opens the Define Threshold Edges dialog window.

_DefineThresholdGa3

Description

 _DefineThresholdGa3();

Opens the Define Threshold dialog window. Click on the help button in the dialog for more information.

_DefineThresholdHsiGa3

Description

 _DefineThresholdHsiGa3();

Opens the Define Threshold dialog window with the HSI method selected as default. Click on the help button in the dialog for more information.

_DefineThresholdIHS

Description

 _DefineThresholdIHS();

This function shows a dialog box to define and perform threshold segmentation with HSI method selected as default.

Note

This function is used for backward compatibility.

_DefineThresholdIntGa3

Description

 _DefineThresholdIntGa3();

Opens the Define Threshold dialog window with the Intensity method selected as default. Click on the help button in the dialog for more information.

_DefineThresholdMchGa3

Description

 _DefineThresholdMchGa3();

Opens the Define Threshold dialog window with the Multi-Channel method selected as default. Click on the help button in the dialog for more information.

_DefineThresholdRgbGa3

Description

 _DefineThresholdRgbGa3();

Opens the Define Threshold dialog window with the RGB method selected as default. Click on the help button in the dialog for more information.

_DetectAllBinary

This function runs the Binary > Smart Threshold command.

Description

 _DetectAllBinary();

This function runs an interactive thresholding tool which can threshold similar binary objects at once.

_DetectCirclesHough

Description

 _DetectCirclesHough();

Opens the Detect Circles Hough dialog window used for detecting circles using the Hough transform.

Inv

Inverts the image searching area.

Smooth

Smooths the image before circle detection.

Edge

Check this option when detecting rings.

Radius From

Low range value of the circle radius which will be detected.

Radius To

High range value of the circle radius which will be detected.

Note

Setting this value very far apart from the From value may be very time consuming for the detecting.

Response

This slider influences the roundness of the detected circles. The higher the value, the more circular the resulting detection.

Accomodate circles to BestFit

This function fine-tunes the detected circles and moves their border to copy the exact path of the circle in the image.

Apply To

Defines the dimension on which the detection will be performed.

_DetectComponentBinary

Description

 _DetectComponentBinary();

This function runs an interactive thresholding tool which can threshold one object.

See Also
_DetectAllBinary

_DetectLines

Description

 _DetectLines();

Opens the Detect Lines dialog window used for detecting straight lines.

See Also

_DetectLinesHough

_DetectLinesHough

Description

 _DetectLinesHough();

Opens the Detect Lines Hough dialog window used for detecting longer straight lines using the Hough transform.

Edge Width

Edge detector size.

Threshold

Standard detection threshold.

Minimal Edge Length

Minimal length of a line which will be detected and marked as binary.

Apply To

Defines the dimension on which the detection will be performed.

See Also

_DetectLines

_GA3_SpotDetection

Description

 _GA3_SpotDetection(
   int  Inverted
);

Opens the GA3 3D Spot Detection window in the selected color detection mode.

Parameters

int Inverted

Color detection method.

0	Bright Spot.
1	Dark Spot.

_GBasedSegmentation

This function runs the Binary > Segmentation > Graph Cut Segmentation command.

Description

 _GBasedSegmentation();

Opens the Graph Cut Segmentation dialog window.

See Also

GBasedSegmentation

_HomogeneousSegmentation

This function runs the Binary > Segmentation > Homogeneous Area Detection command.

Description

 _HomogeneousSegmentation();

Opens the Homogeneous Area Segmentation dialog window.

_SpotDetection

Description

 _SpotDetection(
   int  Inverted
);

This function opens the Spot Detection dialog window.

Parameters

int Inverted

Color detection method.

0	Bright Spot.
1	Dark Spot.

See Also
SpotDetection

_SpotDetection3DLoadSettings

Description

 _SpotDetection3DLoadSettings();

Opens the Load Spot Detection Settings dialog window used for importing the 3D Spot Detection settings from a .spotdetection3D file.

_SpotDetection3DSaveSettings

Description

 _SpotDetection3DSaveSettings();

Saves the 3D Spot Detection dialog window settings into a .spotdetection3D file.

_SpotDetectionLoadSettings

Description

 _SpotDetectionLoadSettings();

Opens the Load Spot Detection Settings dialog window used for importing the Spot Detection settings from a .spotdetection file.

_SpotDetectionSaveSettings

Description

 _SpotDetectionSaveSettings();

Opens the Save Spot Detection Settings dialog window used for exporting the Spot Detection settings to a .spotdetection file.

_Threshold

Description

 _Threshold();

This function equals the Threshold function except when performed on ND2 document you will be prompt to select whether to process the current image, selected dimension, or the whole ND document.

_ThresholdCalcComp

Description

 _ThresholdCalcComp();

This function thresholds the calcium component.

_ThresholdIHS

Description

 _ThresholdIHS();

Performs threshold in the HSI (Hue Saturation Intensity) color space.

_ThresholdLoadSettings

Description

 _ThresholdLoadSettings();

This function loads the current threshold settings from a file. The function displays the Load Threshold Settings dialog window.

_ThresholdOtsu

This function runs the Binary > Auto Threshold command.

Description

 _ThresholdOtsu();

Displays the Auto Threshold dialog window which sets the parameters for the ThresholdOtsu function.

_ThresholdSaveSettings

Description

 _ThresholdSaveSettings();

This function saves the current threshold settings to a file. The function displays the Save Threshold Settings dialog window.

_WoundSegmentation

This function runs the Binary > Segmentation > Wound Area Detection command.

Description

 _WoundSegmentation();

This function opens the Wound Area Detection dialog window.

_WoundSegmentation3D

Description

 _WoundSegmentation3D();

This function opens the Wound area detection in Time dialog window used for wound area detection on time lapses.

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