_LoadMolecules, _MoleculesToRaster, _NSTORMAcquisition, _NSTORMAnalysisNew, _NSTORMLayersAutocorrelate, _NSTORMRender, _NSTORMRenderVolume, DeleteMolecules, LoadMolecules, MoleculesSnapshot, NSTORMRender, NSTORMRenderVolume, NSTORMSetLightPath, NSTORMSetMultilaser, SaveMolecules, ShowMoleculeColorScale

DeleteMolecules

Description 

 DeleteMolecules();

Deletes the molecule data.

LoadMolecules

Description 

 LoadMolecules(
   char *Filename,
   double  PhotonsPerCount,
   char *ChannelMapping
);

Opens the molecule analysis file previously created by the SaveMolecules function.

Parameters

char *Filename

The path and the name of the file.

double PhotonsPerCount

How many photons fall on the detected electrons. Default value is 0.18.

char *ChannelMapping

Assigns molecules to image channels.

MoleculesSnapshot

Description 

 MoleculesSnapshot();

Creates a new molecule document containing only the currently visible (filtered) molecules.

NSTORMRender

Description 

 NSTORMRender(
   double  CalXY,
   double  CalZ,
   int  RenderFlags
);

Renders molecules into an RGB image.

Parameters

double CalXY

XY Calibration in µm/px.

double CalZ

Z Step in µm.

int RenderFlags

This parameter is for future use.

NSTORMRenderVolume

Description 

 NSTORMRenderVolume(
   double  CalXY,
   double  CalZ,
   double  RenderVolumeRadius,
   double  RenderVolumeISO,
   int  RenderFlags
);

Renders molecules into a 3D binary.

Parameters

double CalXY

XY Calibration in µm/px.

double CalZ

Z Step in µm.

double RenderVolumeRadius

Size of the binary sphere around each molecule [µm].

double RenderVolumeISO

This parameter is for future use.

int RenderFlags

This parameter is for future use.

NSTORMSetLightPath

Description 

 NSTORMSetLightPath(
   int  STORMSetlightpath
);

Specifies the light path used for acquiring a STORM image.

Parameters

int STORMSetlightpath

Light Path ID.

NSTORMSetMultilaser

Description 

 NSTORMSetMultilaser(
   char *STORMMultilaser
);

Specifies the illumination device used for acquiring a STORM image.

Parameters

char *STORMMultilaser

Name of the illumination device (name shown in the Device Manager) used for STORM image acquisition.

SaveMolecules

Description 

 SaveMolecules(
   char *Filename,
   int  OutputMode
);

Saves the detected molecules to a file.

Parameters

char *Filename

The path and the name of the file.

int OutputMode

Specifies the molecules output mode.

0	Export to .txt
1	Export to binary M425 (subset of N300)
2	Export to binary N300 (extensive)

ShowMoleculeColorScale

Description 

 ShowMoleculeColorScale(
   int  ShowMolColorScale
);

Displays the molecule color scale.

Parameters

int ShowMolColorScale

Turns on/off the molecule color scale.

0	Scale OFF.
1	Scale ON.

_LoadMolecules

Description 

 _LoadMolecules(
   char *Filename,
   char *BigDocName
);

Loads a molecule file created by the SaveMolecules function as a new image file.

Parameters

char *Filename

The path and the name of the file.

char *BigDocName

Name of the file into which the molecules will be imported.

_MoleculesToRaster

This function runs the Edit > Create View Snapshot from Molecules menu command.

Description 

 _MoleculesToRaster();

Opens the Create raster document from molecules dialog window used for creating a custom raster document from the currently opened molecule image.

_NSTORMAcquisition

This function runs the Applications > N-STORM > Define/Run N-STORM Acquisition menu command.

Description 

 _NSTORMAcquisition(
   int  STORMGUIType
);

Opens the N-STORM acquisition dialog window. Please see Additional Modules > N-STORM > N-STORM Acquisition.

Parameters

int STORMGUIType

This parameter is for future use.

_NSTORMAnalysisNew

This function runs the Applications > N-STORM > N-STORM Offline Analysis Module menu command.

Description 

 _NSTORMAnalysisNew(
   int  STORMFileName
);

Opens the STORM image inside the N-STORM Analysis Module within NIS-Elements.

Parameters

int STORMFileName

Path to the STORM nd2 file.

_NSTORMLayersAutocorrelate

Description 

 _NSTORMLayersAutocorrelate();

Runs the autocorrelation for layer alignment of localization data. It offsets X, Y, Z molecules localized on successive Z stack layers.

_NSTORMRender

Description 

 _NSTORMRender();

Opens the Render Volume Settings dialog window. See NSTORMRender.

_NSTORMRenderVolume

Description 

 _NSTORMRenderVolume();

Opens the Render Volume Settings dialog window. See NSTORMRenderVolume.