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_CallLeonardo, _CreateWidefieldFromSIM, _GPU_EDF_CreateEDFImage, _GPU_HDR_CreateHDRImage, _HDR_CaptureHDRImage, _HDR_CreateHDRImage, _ND_StitchMultipoint, _ND_StitchMultipointGa3, _SelectBestFocusedFromND, _SelectFocusedCriterion, AlignCurrentNDDocument, AlignCurrentNDDocumentRect, CreateWidefieldFromSIM, Denoise3DCurrentNDDocument, GPU_EDF_CreateEDFImage, GPU_HDR_CreateHDRImage, ND_StitchMultipoint, ND_StitchMultipointGa3, SelectBestFocusedFromND, ShowFocusedGraphFromND, STORMVV_ImportMoleculeList

AlignCurrentNDDocument

This function runs the Image > ND Processing > Align Current ND Document command.

Description

 AlignCurrentNDDocument();

This function aligns frames of an ND document.

AlignCurrentNDDocumentRect

Description

 AlignCurrentNDDocumentRect(
   int  AlignCurrentND_Left,
   int  AlignCurrentND_Top,
   int  AlignCurrentND_Right,
   int  AlignCurrentND_Bottom,
   int  AlignCurrentND_Axis,
   int  AlignCurrentND_Method,
   int  AlignCurrentND_AlignTo,
   int  AlignCurrentND_Blurr,
   int  AlignCurrentND_FavorSmooth,
   int  AlignCurrentND_UseSR
);

This function aligns the frames of the current ND document. Define a rectangular area of the current frame which does not change (is identical) throughout the whole stack of images. The algorithm will match all images according to this area.

Parameters

int AlignCurrentND_Left

The distance in X direction of the left side of the area [px].

int AlignCurrentND_Top

The distance in Y direction of the top side of the area [px].

int AlignCurrentND_Right

The distance in X direction of the right side of the area [px].

int AlignCurrentND_Bottom

The distance in Y direction of the bottom side of the area [px].

int AlignCurrentND_Axis

ND dimension to be Aligned

int AlignCurrentND_Method

The method of cropping after the frames are shifted.

0	Intersection - reduces the document size.
1	Original size, preserves the original ND document dimensions.
2	Union - adds borders to frames.

int AlignCurrentND_AlignTo

ND document alignment processing method.

0	Previous Frame
1	First Frame

int AlignCurrentND_Blurr

Equals the Use correction for image heavily corrupted by noise command.

0	Doesn't apply blur processing.
1	Applies blur processing.

int AlignCurrentND_FavorSmooth

Equals the Favor correction of the smooth movement command.

0	Off
1	On

int AlignCurrentND_UseSR

Equals the Use SuperResolution aligment command.

0	Off
1	On

GPU_HDR_CreateHDRImage

Description

 GPU_HDR_CreateHDRImage(
   char *HDR_CRI_StartDir,
   char *HDR_CRI_Files
);

Creates an HDR image using GPU.

Parameters

char *HDR_CRI_StartDir

A path to the directory where the images are stored.

char *HDR_CRI_Files

The list of image files separated by a semicolon. The images must equal in size (resolution).

GPU_EDF_CreateEDFImage

Description

 GPU_EDF_CreateEDFImage(
   char *HDR_CRI_StartDir,
   char *HDR_CRI_Files
);

Creates an EDF image using GPU.

Parameters

char *HDR_CRI_StartDir

A path to the directory where the images are stored.

char *HDR_CRI_Files

The list of image files separated by a semicolon. The images must equal in size (resolution).

CreateWidefieldFromSIM

Interactive command to this function: Applications > N-SIM > Create Widefield From SIM

Description

 CreateWidefieldFromSIM(
   int  TilesInX,
   int  TilesInY
);

Creates a widefield image from a SIM image.

Parameters

int TilesInX

Number of tiles in X.

int TilesInY

Number of tiles in Y.

_CreateWidefieldFromSIM

This function runs the Applications > N-SIM > Create Widefield From SIM command.

Description

 _CreateWidefieldFromSIM();

Opens the Create Widefield from SIM dialog window enabling to create an averaged widefield image from a SIM image.

See Also
CreateWidefieldFromSIM

Denoise3DCurrentNDDocument

Description

 Denoise3DCurrentNDDocument(
   int  DenoisePB1Power,
   int  DenoisePB2Power,
   int  DenoisePB3Power,
   int  DenoisePB4Power,
   int  DenoisePB5Power,
   int  DenoisePB6Power,
   int  DenoisePB7Power,
   int  DenoisePB8Power,
   int  AlignCurrentND_Axis
);

Performs 3D Denoise on the current ND document.

Parameters

int DenoisePB1Power

strength of the denoising algorithm on the first channel

int DenoisePB2Power

strength of the denoising algorithm on the second channel

int DenoisePB3Power

strength of the denoising algorithm on the third channel

int DenoisePB4Power

strength of the denoising algorithm on the fourth channel

int DenoisePB5Power

strength of the denoising algorithm on the fifth channel

int DenoisePB6Power

strength of the denoising algorithm on the sixth channel

int DenoisePB7Power

strength of the denoising algorithm on the seventh channel

int DenoisePB8Power

strength of the denoising algorithm on the eight channel

int AlignCurrentND_Axis

ND dimension to be Aligned

ND_StitchMultipoint

Interactive command to this function: Image > ND Processing > Stitch Multipoint to Large Image

Description

 ND_StitchMultipoint(
   int  StitchNDMethod,
   int  StitchNDPostProcess,
   int  StitchNDShadingType,
   int  StitchNDShadingOffset,
   int  StitchNDRegistration
);

This function enables you to stitch frames of an XY (multi-point) ND image and create a large image.

Parameters

int StitchNDMethod

Select the method used to stitch neighboring (and overlapping) images.

0	Blending - images will be blended into each other.
1	Optimal path - the algorithm computes a path between the images where both images are least different and removes the outer areas.

int StitchNDPostProcess

Apply post-processing (shading correction and dust-removal)

0	No post-processing.
1	Apply at least the shading correction.

int StitchNDShadingType

Select the type of image you will be processing.

0	Brightfield
1	DIC-like
2	Fluorescence (with offset)

int StitchNDShadingOffset

Set the offset value to be subtracted from the image before applying the fluorescence correction.

int StitchNDRegistration

Enable registration of the overlapping images? If images do not overlap, this parameter is ignored.

0	Image registration OFF.
1	Image registration ON.

ND_StitchMultipointGa3

Description

 ND_StitchMultipointGa3(
   int  iStitchingMethod,
   int  iRegistration,
   int  iAutoShadingCorrection,
   int  iType,
   int  NDDimension
);

Stitches frames of the connected image into a large image.

Parameters

int iStitchingMethod

Stitching method.

0	Blending
1	Optimal Path

int iRegistration

Image registration (precise stitching).

0	Off
1	On

int iAutoShadingCorrection

Automatic shading correction.

0	Off
1	On

int iType

Method type (depends on the particular function).

int NDDimension

Frame selection of the ND document.

0	Current frame
1	Time dimension
2	Multipoint dimension
4	Z dimension
15	All frames (the whole ND document).
16	Selection - the frames/dimensions selected by mouse within the ND control bar will be affected.

Note

Value combination is also possible (e.g.: M+T=3, Z+T=5, M+Z=6).

STORMVV_ImportMoleculeList

This function runs the Applications > Single Particle Tracking > Import Molecule List command.

Description

 STORMVV_ImportMoleculeList();

Opens a dialog window for importing a molecule list from a *.roi file.

SelectBestFocusedFromND

Interactive command to this function: Image > ND Processing > Select Best Focused Plane

Description

 SelectBestFocusedFromND(
   int  FocusCriterion,
   char *ComponentMask
);

This function examines the current ND2 file and selects the Z plane which is focused best. You will be prompt to select the method which will determine the focus criterion (the same one used by the StgFocusSetCriterion function). A new ND2 image is created by deleting the rest of the Z planes.

Parameters

int FocusCriterion

Use focus criterion

0	bright field - standard contrast based criterion
1	intensity - criterion independent on pixel intensity
2	fluorescence - suitable for fluorescence microscopy
3	noise-resistant - estimates the noise level in the image and ignores it
4	confocal - criterion is based only on the intensity values, can be useful in confocal microscopy

char *ComponentMask

Component mask.

0	Component not selected.
1	Component selected.

See Also
StgFocusSetCriterion

ShowFocusedGraphFromND

Description

 ShowFocusedGraphFromND(
   int  ShowBestFocusPlaneGraph
);

Creates the Best Focus Planes graph. Frames with higher y axis values are more in focus.

Parameters

int ShowBestFocusPlaneGraph

Displays the best focus plane graph.

_CallLeonardo

This function runs the Applications > Alveole Leonardo > Show Alveole Leonardo Dialog command.

Description

 _CallLeonardo();

Opens the Alvéole Leonardo external application.

_GPU_EDF_CreateEDFImage

Description

 _GPU_EDF_CreateEDFImage();

Opens the Select EDF source files where the user selects files for the EDF image which is subsequently created using GPU.

_GPU_HDR_CreateHDRImage

Description

 _GPU_HDR_CreateHDRImage();

Opens the Select HDR source files where the user selects files for the HDR image which is subsequently created using GPU.

_HDR_CaptureHDRImage

This function runs the Applications > HDR > Capture HDR Image command.

Description

 _HDR_CaptureHDRImage();

_HDR_CreateHDRImage

This function runs the Applications > HDR > Create HDR Image from File command.

Description

 _HDR_CreateHDRImage();

_ND_StitchMultipoint

This function runs the Image > ND Processing > Stitch Multipoint to Large Image command.

Description

 _ND_StitchMultipoint();

This function opens a dialog window which can be used to stitch a multi-point ND image to a single large image.

_SelectBestFocusedFromND

This function runs the Image > ND Processing > Select Best Focused Plane command.

Description

 _SelectBestFocusedFromND();

Opens the Select Best Focus Plane dialog window.

See Also
SelectBestFocusedFromND

_SelectFocusedCriterion

Description

 _SelectFocusedCriterion();

Opens the Select Focus Criterion dialog window used for setting the focus criterion.

See Also

SelectBestFocusedFromND

_ND_StitchMultipointGa3

Description

 _ND_StitchMultipointGa3();

Opens the ND_StitchMultipoint dialog window used for stitching frames of the connected image into a large image.

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