What is FRET?
A non-radiative transfer of energy from a donor molecule to an acceptor molecule.
Förster/Fluorescence Resonance Energy Transfer
Fluorescence emission from an acceptor molecule due to non radiative energy transfer when a donor molecule was excited.
Conditions for FRET
Donor's emission must overlap acceptor's excitation
Donor and acceptor molecules must be <100 Ã… (angstroms) apart.
Donor and acceptor molecules' dipole orientation must be parallel.