During the scanning process you can follow the sequence of icons in the left tool bar.
Before you start the scanning, please make sure the camera and objective settings are properly calibrated - see System settings.
Optimal illumination should be set before analyzing any filter. Move the XY stage to the position, where the analyzed part of the filter is well visible. Software can assist you in checking and setting the optimal illumination. Click
Adjust Light to show the Check Illumination dialog window. Mean Intensity and the Overexposure Ratio characterize the scene and show recommended values. In case you decide to use this routine, you need to setup the recommended intensity and over-illumination tolerance to be compliant with your system. This is done in the Scanning tab of the 
Options dialog (see Advanced Scanning).
Figure 609.
Click
Define Position to start defining the filter position. Follow the instructions shown in the window. Enter the diameter of the scanned filter. Current objective calibration is shown below.
Figure 610.
Move the XY stage until the crosshair touches the left filter boundary. Then click .
Figure 611.
Click
Check Positions to check the filter edges. XY stage will reach the left filter edge and the following window will show up. Also a crosshair is shown in the camera image. It should cross the edge of the filter. If it does, click . If not, move the stage or drag the crosshair by mouse, so that the crosshair crosses the filter edge and click - the coordinates will be redefined to fit the point. Follow the instructions to check the filter edges.
Figure 612.
Now move on to Scanning itself. By default, no autofocus is used during the scanning. If needed, go to the Focusing tab of the
Options (see Autofocus during scanning).Click
Scan Filter to start scanning the filter.Note
If the calibration in use is different from the recommended one, a warning window appears. You can set the calibration again (by selecting the correct active objective) or just click if you are satisfied with it and the recommended calibration will be set to the current value.
Note
If there is an opened file in the Filter Preview window, the
button will be disabled. In such case, click 
to close this file.Note
It is possible to scan the filter and immediately analyze it by clicking
Scan and Measure.The following window appears:
Figure 613. Scanning Progress dialog window
This dialog displays the scanning progress, information about the ratio of overexposed pixels and mean intensity. Pause the scanning by the
button or quit the procedure by the 
button at any time.Caution
If a continuous automatic exposure function of your camera is turned on, it will be automatically turned off until the scanning is finished. This is to prevent single tiles of the scanned filter to differ in brightness.
The filter is saved to the temporary folder by default, but it is possible to select a permanent storage to a defined folder (see point number 10). Files are saved as a .flt. file format which contains the image data of all the fields, scanning date, pixel calibration, real filter dimensions and measurement circle, number of fields, images, dimensions, and other morphological data of all measured and edited particles.