Custom equations can be effectively used to enhance your captured / live images. To add a custom equation to a live image, run Applications > Ratio, Ca2+, FRET > Add New Equation View on Live. The following sections show some common and useful custom equations.
The equation Current is just a simple copy of the current image / channel. Applying custom LUTs only to this image can help with the visualization.
Figure 667.
Equation -Current simply inverts the current image / channel. Often it is easier to see small details in the inverted mode.
Figure 668.
Equation Current-Previous displays only moving objects in black and white colors. The distance moved per frame can be easily seen - white shows the current position and black is the previous position. Static objects are removed from the image.
Figure 671.
This equation can be modified to see only moving objects in white color: Average[+4..+6]-Average[+0..+3]. Here average is used for image smoothing.
Figure 672.
Another way to hide static objects and display only the moving ones is to subtract average of all frames from the current frame. Use this equation: Current-Average. This can also shade-correct the image in some cases.
Figure 673.
