Real Time EDF

(requires: EDF Module)

Having a motorized Z drive, you can use the following procedure to capture an EDF image:

  1. Run the Applications > EDF > Real Time EDF command. The Real Time EDF window appears with the Align images check box added.

  2. Select whether to Align images before creating the EDF image or not.

  3. If you want to use high dynamic range, check Use HDR and set its parameters (see: Applications > HDR > Capture HDR Image).

  4. Choose whether to create a Z-Map (Balanced, Correlation or Original) and choose the Z-Profile coordinates (Zero Based or Real Coordinates). If the Z-map calculation is not necessary for your task, select None in the drop-down menu to speed up the EDF image acquisition.

  5. Set the parameters of the Z Series Acquisition.

    See: Z-series Acquisition.

  6. Click Run.

Note

Either before or after the capture you can execute a Command (from the Command List) or run a Macro (from a file).