Browsing Results

When the Scanning phase is finished, the results are automatically opened or can be opened manually. The results layout is designed specifically for each assay and usually consists of five main parts (from left to right):

  • Assay Explorer - result list for the current group which can be arranged by Date, by Experiment (Name field in the Insert Plate phase), by Assay or by User.

  • Well plate view

  • Well plate results (graph and table)

  • Single well preview

  • Single well results (graph and table)

Main Toolbar

Hide

Hides the Assay Explorer.

Home

Returns to the NIS-Elements SE home page.

Run Analysis

This button is active only if the analysis on the current assay was not executed yet. Use it to run the analysis afterwards.

Adjust Analysis Parameters

Some assays may have adjustable parameters which can be adjusted here.

Edit Labeling & Dosing

Opens the Labeling and Dosing dialog used for adjusting the labeling and dosing.

Repeat Acquisition with same settings

Runs acquisition with the same settings all over again.

Well plate view

Image

Each well in the well plate view is filled with the acquired image.

Labeling

Displays color labels for each well if the labels were defined.

Dosing

Displays dosing concentrations if they were defined.

Heatmap

Data of the selected feature is represented by a color square.

Bars

Data of the selected features are shown as bars.

Barstack

Data of the selected features are displayed as bars stacked one over the other.

Boxplot

Data of the selected feature are shown as a box and whisker plot.

Violin

Data of the selected feature are shown as violin charts.

Data

Feature used for the well plate view visualization.

Show numbers in wells

Feature value is shown directly inside each well.

Shows a scale bar in the well plate view.

Well plate results

Shows results from all measured features for the whole well plate.

Dose-response

Shows the dose-response curve describing the magnitude of the response of an organism as a function of exposure to a chemical after a certain exposure time.

Notes

Can be used to insert additional notes related to the current well plate.

Report

Generates a detailed predefined report already customised for each specific assay.

Fit equation

Selects the features for which the dose response curve is calculated.

Error Bars Visible

Displays the error bars (graphical representations of the variability of data) in the chart.

Legend Visible

Displays the graph legend.

Summary

Shows the summary information about the measurement.

Data table

Shows a table with the well plate measurement data.

Auto size

Automatically sizes the table column width.

Statistics

Shows the min, max and mean statistics below the table.

Pan

Pan (move) tool to be used inside the graph.

Wheel Zoom

Switches the mouse wheel to be used for zooming in the graph.

Reset

Resets the graph.

Single well preview

Preview image is shown for the well selected in the top-left well plate view.

Auto LUTs

Adjusts the look-up-tables with the purpose to enhance the image reasonably. Context menu over this button reveals the Auto Scale Settings window where the Low and High fields determine how many of all pixels of the picture are left outside the LUTs sliders when Auto LUTs is applied (0-10%). It also adjusts the opacity of the brightfield channel (Brightfield Opacity).

Split Components

Turns on a special view, where color channels of the image are displayed separately (tiled).

Show Scale

Displays a line scale which can be moved around the image using the mouse. Context menu over the scale enables the user to Burn Scale into the image permanently, move it to the Default Scale Position, Lock Scale Position or adjust Scale Properties (View > Layers Properties > Scale Properties).

Tip

Use / to show or hide the scale.

Show Graticule

Displays a graticule layer over the image for quick and approximate measurements. Graticules behave like adjustable floating rulers. User can simply align a graticule with the measured object and read the distance value. Click on the line and drag it to move the graticule. Context menu over the graticule enables the user to move the graticule to the center (Move to Center) and adjust the Graticule Properties.

Tip

Use the * key shortcut to show or hide the graticule layer.

Show Binary

Displays or hides the binary layer in the image. Use the button below the single well preview to open the binary layer opacity panel where you can adjust the color and intensity for each binary layer present in the image.

Tip

Use Ctrl + Up/Down to decrease/increase the binary layer transparency.

Fit to Screen

Adjusts the zoom factor to view exactly the whole image as big as possible.

Zoom 1:1

Adjusts the zoom factor so that one pixel of the image matches one pixel of your monitor.

Zoom

Select a zoom factor from the drop-down menu.

Single well results

Data are shown for the well selected in the top-left well plate view.

Scatterplot

Well data are shown in a scatter plot.

Histogram

Well data are shown in a histogram.

Bars

Well data are shown in a bar chart.

X/Y/Z axis feature

Choose a feature for each available axis (depends on the selected graph).

Fit normal distribution

Adds the normal trend line to the graph.

Scale to fit the data

Fits the data into the graph area.

Auto size

Automatically sizes the table column width.

Statistics

Shows the min, max and mean statistics for the single well right below the table.