When the Scanning phase is finished, the results are automatically opened or can be opened manually. The results layout is designed specifically for each assay and usually consists of five main parts (from left to right):
Assay Explorer - result list for the current group which can be arranged by Date, by Experiment (Name field in the Insert Plate phase), by Assay or by User.
Well plate view
Well plate results (graph and table)
Single well preview
Single well results (graph and table)
Main Toolbar
Hide Hides the Assay Explorer.
Home Returns to the NIS-Elements SE home page.
Run Analysis This button is active only if the analysis on the current assay was not executed yet. Use it to run the analysis afterwards.
Run Analysis on Cluster Processes the analysis in the dedicated cloud cluster of computers. This button is shown only when Run analysis is switched off in the assay settings and the Cluster computing is set (in the Assay User Management) and successfully connected.
Adjust Analysis Parameters Some assays may have adjustable parameters which can be adjusted here.
Edit Labeling & Dosing Opens the Labeling and Dosing dialog used for adjusting the labeling and dosing.
Repeat Acquisition with same settings Runs acquisition with the same settings all over again.
Well plate view
Image Each well in the well plate view is filled with the acquired image.
Labeling Displays color labels for each well if the labels were defined.
Dosing Displays dosing concentrations if they were defined.
Heatmap Data of the selected feature is represented by a color square.
Bars Data of the selected features are shown as bars.
Barstack Data of the selected features are displayed as bars stacked one over the other.
Linechart Data of the selected timelapse features are displayed as line charts.
Boxplot Data of the selected feature are shown as a box and whisker plot.
Violin Data of the selected feature are shown as violin charts.
Linechart Data of the selected feature are shown as line charts (y as function of x).
Color Axis Feature Selects the feature which will be visualized using a color gradient.
Size Axis Feature Selects the feature which will be visualized using the size.
Feature used for the well plate view visualization.
Show numbers in wells Feature value is shown directly inside each well.
Scale visible Shows a scale bar in the well plate view.
Well plate results
Shows results from all measured features for the whole well plate.
Dose-response Shows the dose-response curve describing the magnitude of the response of an organism as a function of exposure to a chemical after a certain exposure time.
Notes Can be used to insert additional notes related to the current well plate.
Report Generates a detailed predefined report already customised for each specific assay.
Selects the features for which the dose response curve is calculated.
Error Bars Visible Displays the error bars (graphical representations of the variability of data) in the chart.
Legend Visible Displays the graph legend.
Summary Shows the summary information about the measurement.
Data table Shows a table with the well plate measurement data.
Auto size all columns Automatically sizes the table column width.
Statistics Shows the min, max and mean statistics below the table.
Export Exports current objects or all objects to the selected file format.
Pan Pan (move) tool for positioning the graph.
Select Frame By clicking in the graph the dimension frame navigation changes accordingly to the vertical line. It also shows tooltip information about the current frame.
Lasso Select Click and drag inside the graph (draw any shape) to select the data.
Box Select Click and drag a rectangle in the graph to select the data.
Horizontal Pan Pans (moves) the graph horizontally.
Wheel Zoom Switches the mouse wheel to be used for zooming in the graph.
Tap Activate this function and click inside the graph to select a data point.
Hover Displays information about the data point(s) under the cursor.
Reset Resets the graph.
Export Exports the graph into a .png image.
Copy to Clipboard Copies the graph image into clipboard.
Single well preview
Preview image is shown for the well selected in the top-left well plate view.
Auto LUTs Adjusts the look-up-tables with the purpose to enhance the image reasonably. Context menu over this button reveals the Auto Scale Settings window where the Low and High fields determine how many of all pixels of the picture are left outside the LUTs sliders when Auto LUTs is applied (0-10%). It also adjusts the opacity of the brightfield channel (Brightfield Opacity).
Split Components Turns on a special view, where color channels of the image are displayed separately (tiled).
Show Scale Displays a line scale which can be moved around the image using the mouse. Context menu over the scale enables the user to Burn Scale into the image permanently, move it to the Default Scale Position, Lock Scale Position or adjust Scale Properties (
View > Layers Properties > Scale Properties).
Tip
Use / to show or hide the scale.
Show Graticule Displays a graticule layer over the image for quick and approximate measurements. Graticules behave like adjustable floating rulers. User can simply align a graticule with the measured object and read the distance value. Click on the line and drag it to move the graticule. Context menu over the graticule enables the user to move the graticule to the center (Move to Center) and adjust the Graticule Properties.
Tip
Use the * key shortcut to show or hide the graticule layer.
Show Binary Displays or hides the binary layer in the image. Use the
Binary Layers button below the single well preview to open the binary layer opacity panel where you can adjust the color and intensity for each binary layer present in the image.
Tip
Use Ctrl + Up/Down to decrease/increase the binary layer transparency.
Fit to Screen Adjusts the zoom factor to view exactly the whole image as big as possible.
Zoom 1:1 Adjusts the zoom factor so that one pixel of the image matches one pixel of your monitor.
Select a zoom factor from the drop-down menu.
Select All Frames Selects all frames of the dimension.
First Frame Jumps to the first frame of the selected dimension.
Previous Position (Left) Displays the previous frame of the dimension.
Play Sequence Plays all images of the dimension at a defined speed. If a selection is applied, only the selected images are included in the playback.
Stop Playing Stops playing the sequence at the last displayed frame.
Next Position (Right) Displays the next frame of the dimension.
Last Frame Jumps to the last frame of the selected dimension.
Advanced Options Reveals/hides the speed controls described below.
Decrease Playing Speed Changes the playback speed by one step down.
Click in the bar to define the speed using the mouse.
Increase Playing Speed Changes the playback speed by one step up.
Real Time Playing Speed Sets the playing speed to real-time (as the image was captured).
Maximum Playing Speed for Every Frame Sets the playing speed to maximum while the display of every frame is guaranteed (when the speed is set to maximum by the + button, some frames are usually omitted when playing the sequence depending on your graphics card).
Single well results
Data are shown for the well selected in the top-left well plate view.
Scatterplot Well data are shown in a scatter plot.
Histogram Well data are shown in a histogram.
Barchart Well data are shown in a bar chart.
Linechart Well data are shown as a line chart.
Fitplot Well data are shown as a fitted curve.
Choose a feature for each available axis (depends on the selected graph).
Fit normal distribution Adds the normal trend line to the graph.
Scale to fit the data Fits the data into the graph area.
Auto size Automatically sizes the table column width.
Statistics Shows the min, max and mean statistics for the single well right below the table.