Open the image file to be processed.
Select one of the deconvolution commands and select the deconvolution method. See Choosing the Deconvolution Method.
Adjust the PSF parameters, eventually use the button and select the file containing the PSF image. See Determining the Point Spread Function (PSF).
Adjust the parameters which are not part of PSF such as number of iterations, Z-stack support or level of noise.
Select image channels which will be processed.
Run deconvolution.
Bit-depth conversion
Deconvolution processing can change the range of intensity values to compensate for the higher dynamics of intensities in the resulting image. Bit-depth conversion is applied in such cases. After resetting LUTs the image can look darker than expected because the distribution of intensity values is having a different ratio.
Images having bit-depth smaller than 16-bit are converted to 16-bit, while images already having 16-bits are converted to floating point image. This applies to 2D Automatic deconvolution. Other 2D deconvolution methods keep their original bit-depth. 3D deconvolution methods use bit-depth conversion (<16-bit to 16-bit and 16-bit to float) if Keep Current Document is selected. The deconvolved image is always converted to floating point image if Create New Document is selected.