The Smart Experiment (SE) module was specifically designed for the Nikon Ji microscope with the aim of streamlining the process of well plate imaging using predefined assays. The module utilizes artificial intelligence to automatically detect well plates, a task that previously required a manual input. Additionally, it incorporates the power of Autosignal.ai, an intelligent algorithm that determines the optimal brightness values for well plates and AI-based automatic objective focus.
The module comes with 5 default assays that have been established as scientifically accurate. All assays are designed to save user time and effort by automatically identifying the well plate type, cellular staining material used with each assay, by measuring only the relevant parameters and by generating a comprehensive report. This level of automation not only improves the precision and accuracy of the data, but also highly reduces the potential for user input errors. For those who require additional assays, the module offers 8 more assays that can be purchased.
Default assays
Cell Counting - endpoint
Cytotoxicity
Intensity Measurement
Size & Morphological analysis
Transfection Efficiency
Additional assays
Apoptosis
Autophagy
DNA Damage - gammaH2AX
Endocytosis
Micronucleus Test
Mitochondrial Toxicity
Nuclear Translocation
Phagocytosis
Running NIS-Elements SE opens the application home page with 4 main buttons:
Figure 1580. SE main buttons.
Opens the Assay layout guiding the user through the process of selecting the appropriate assay, configuring its settings, selecting the relevant wells and labeling or dosing the sample.
Shows detailed results for both the well plate and individual wells. The results view is specifically designed for each assay, ensuring that the user receives precise and relevant information for their particular experiment.
Opens the Assay Definition window where an assay can be enabled and its parameters adjusted for a later run in the Run Assay window. A copy of an existing assay can be created here as well.
Opens the Assay User Management window used for creating the user groups and assigning user privileges, configuring storage settings and setting up e-mail notifications.